Web of Science: 8 cites, Scopus: 9 cites, Google Scholar: cites,
Changes of salivary biomarkers under different storage conditions : Effects of temperature and length of storage
Barranco, T. (Universidad de Murcia. Interlab-UMU)
Rubio, C.P. (Universidad de Murcia. Interlab-UMU)
Tvarijonaviciute, Asta (Universidad de Murcia. Interlab-UMU)
Rubio, M. (Universidad CEU Cardenal Herrera. Departament de Medicina y Cirugía Animal)
Damia, E. (Universidad CEU Cardenal Herrera. Departament de Medicina y Cirugía Animal)
Lamy, E. (Universidad de Evora. Institute of Mediterranean Agricultural and Environmental Sciences (ICAAM))
Cugat, R. (Arthroscopy and Traumatology Unit of the Quirón Hospital)
Cerón, J.J. (Universidad de Murcia. Interlab-UMU)
Tecles, F. (Universidad de Murcia. Interlab-UMU)
Escribano Tortosa, Damián (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)

Data: 2019
Resum: Introduction: In this report, we aimed to examine the stability of various analytes in saliva under different storage conditions. Materials and methods: Alpha-amylase (AMY), cholinesterase (CHE), lipase (Lip), total esterase (TEA), creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LD), lactate (Lact), adenosine deaminase (ADA), Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability (FRAS), cupric reducing antioxidant capacity (CUPRAC), uric acid (UA), catalase (CAT), advanced oxidation protein products (AOPP) and hydrogen peroxide (H O) were colorimetrically measured in saliva obtained by passive drool from 12 healthy voluntary donors at baseline and after 3, 6, 24, 72 hours, 7 and 14 days at room temperature (RT) and 4 ºC, and after 14 days, 1, 3 and 6 months at - 20 ºC and - 80 ºC. Results: At RT, changes appeared at 6 hours for TEA and H O; 24 hours for Lip, CK, ADA and CUPRAC; and 72 hours for LD, Lact, FRAS, UA and AOPP. At 4 ºC changes were observed after 6 hours for TEA and H O; 24 hours for Lip and CUPRAC; 72 hours for CK; and 7 days for LD, FRAS and UA. At - 20 ºC changes appeared after 14 days for AST, Lip, CK and LD; and 3 months for TEA and H O. At - 80 ºC observed changes were after 3 months for TEA and H O. Conclusions: In short-term storage, the analytes were more stable at 4 ºC than at room temperature, whereas in long-term storage they were more stable at -80 ºC than at - 20 ºC.
Nota: Número d'acord de subvenció PFST/IF/01778/2013
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: article ; recerca ; publishedVersion
Matèria: Marcadors bioquímics ; Enzyme stability ; Oxidative stress ; Biomarker ; Saliva
Publicat a: Biochemia medica, Vol. 29 Núm. 1 (2019) , p. 10706, ISSN 1846-7482

DOI: 10.11613/BM.2019.010706
PMID: 30799975


18 p, 145.6 KB

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