Magnetic resonance microscopy and correlative histopathology of the infarcted heart
Perez-Terol, Itziar (Instituto de Investigación Sanitaria INCLIVA (València, Comunitat Valenciana))
Rios-Navarro, César (Hospital Clínic Universitari (València))
de Dios, Elena (Hospital Clínic Universitari (València))
Morales, José Manuel (Pathology Department. School of Medicine. University of Valencia)
Gavara Doñate, Jose (Hospital Clínic Universitari (València))
Perez-Sole, Nerea (Hospital Clínic Universitari (València))
Diaz, Ana (Unidad Central de Investigación Biomédica. University of Valencia)
Minana, Gema (Medicine Department. School of Medicine. University of Valencia)
Segura-Sabater, Remedios (Instituto de Investigación Sanitaria INCLIVA (València, Comunitat Valenciana))
Bonanad, Clara (Medicine Department. School of Medicine. University of Valencia)
Bayés-Genís, Antoni (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Husser, Oliver (St. Johannes Hospital(Alemania))
Monmeneu, José V. (Cardiovascular Magnetic Resonance Unit. ERESA)
Lopez-Lereu, María P. (Cardiovascular Magnetic Resonance Unit. ERESA)
Núñez, Julio (Medicine Department. School of Medicine. University of Valencia)
Chorro, Francisco J. (Medicine Department. School of Medicine. University of Valencia)
Ruiz-Sauri, Amparo (Pathology Department. School of Medicine. University of Valencia)
Bodi, Vicente (Medicine Department. School of Medicine. University of Valencia)
Monleon, Daniel (Centro de Investigación Biomédica en Red sobre Fragilidad y Envejecimiento Saludable)
Universitat Autònoma de Barcelona

Data:	2019
Resum:	Delayed enhancement cardiovascular magnetic resonance (MR) is the gold-standard for non-invasive assessment after myocardial infarction (MI). MR microscopy (MRM) provides a level of detail comparable to the macro objective of light microscopy. We used MRM and correlative histopathology to identify infarct and remote tissue in contrast agent-free multi-sequence MRM in swine MI hearts. One control group (n = 3 swine) and two experimental MI groups were formed: 90 min of ischemia followed by 1 week (acute MI = 6 swine) or 1 month (chronic MI = 5 swine) reperfusion. Representative samples of each heart were analysed by contrast agent-free multi-sequence (T1-weighting, T2-weighting, T2*-weighting, T2-mapping, and T2*-mapping). MRM was performed in a 14-Tesla vertical axis imager (Bruker-AVANCE 600 system). Images from MRM and the corresponding histopathological stained samples revealed differences in signal intensities between infarct and remote areas in both MI groups (p-value < 0. 001). The multivariable models allowed us to precisely classify regions of interest (acute MI: specificity 92% and sensitivity 80%; chronic MI: specificity 100% and sensitivity 98%). Probabilistic maps based on MRM images clearly delineated the infarcted regions. As a proof of concept, these results illustrate the potential of MRM with correlative histopathology as a platform for exploring novel contrast agent-free MR biomarkers after MI.
Ajuts:	Instituto de Salud Carlos III PI14-00271 Instituto de Salud Carlos III PI17-01836 Instituto de Salud Carlos III PIE15-00013 Ministerio de Economía y Competitividad SAF2014-52875R Agencia Estatal de Investigación PCIN-2017-117
Nota:	Altres ajuts:The present study was supported by the EU Joint Programming Initiative 'A Healthy Diet for a Healthy Life' (JPI HDHL INTIMIC-085), Generalitat Valenciana (GV/2018/116), INCLIVA and Universitat de Valencia (program VLC-BIOCLINIC 20-nanomIRM-2016A).
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Cardiology ; Diagnostic markers
Publicat a:	Scientific reports, Vol. 9 Núm. 1 (january 2019) , p. 20017, ISSN 2045-2322

DOI: 10.1038/s41598-019-56436-5
PMID: 31882712

10 p, 1.9 MB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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