Use of IP-10 detection in dried plasma spots for latent tuberculosis infection diagnosis in contacts via mail
Villar-Hernández, Raquel (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Latorre, Irene (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
De Souza Galvão, Maria Luiza (Hospital Universitari Vall d'Hebron)
Jiménez, María A. (Hospital Universitari Vall d'Hebron)
Ruiz Manzano, J (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pilarte, Jose (Hospital Universitari Vall d'Hebron)
García-García, Esther (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Muriel-Moreno, Beatriz (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Cantos, Adela (Hospital Universitari Vall d'Hebron)
Altet, Neus (Hospital Universitari Vall d'Hebron)
Millet, Joan-Pau (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
González-Díaz, Yoel D. (Unidad Clínica de Tratamiento Directamente Observado "Serveis Clinics")
Molina-Pinargote, Israel (Unidad Clínica de Tratamiento Directamente Observado "Serveis Clinics")
Prat i Aymerich, Cristina (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Ruhwald, Morten (Statens Serum Institut (Copenhagen, Dinamarca). Department of Infectious Disease Immunology)
Domínguez, José (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Universitat Autònoma de Barcelona

Data:	2019
Resum:	The aim of this study was to test the use of IP-10 detection in dried plasma from contact studies individuals (contacts of smear positive patients), by comparing it with IP-10 and IFN-γ detection in direct plasma, to establish IP-10 detection in DPS as a useful assay for LTBI diagnosis. Whole blood samples were collected from 80 subjects: 12 with active tuberculosis (TB), and 68 from contact studies. The amount of IFN-γ produced by sensitized T cells was determined in direct plasma by QuantiFERON Gold In-Tube test. IP-10 levels were determined in direct and dried plasma by an in-house ELISA. For dried plasma IP-10 determination, two 25 µl plasma drops were dried in Whatman903 filter paper and sent by mail to the laboratory. Regarding TB patients, 100. 0%, 91. 7% and 75. 0% were positive for IFN-γ detection and IP-10 detection in direct and dried plasma, respectively. In contacts, 69. 1%, 60. 3% and 48. 5% had positive results after IFN-γ and IP-10 in direct and dried plasma, respectively. The agreement among in vitro tests was substantial and IP-10 levels in direct and dried plasma were strongly correlated (r = 0. 897). In conclusion, IP-10 detection in dried plasma is a simple and safe method that would help improve LTBI management.
Ajuts:	Instituto de Salud Carlos III PI13-01546 Instituto de Salud Carlos III PI16-01912
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Scientific reports, Vol. 9 Núm. 1 (january 2019) , p. 3943, ISSN 2045-2322

DOI: 10.1038/s41598-019-40778-1
PMID: 30850687

11 p, 1.7 MB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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