Vascular interstitial cells in retinal arteriolar annuli are altered during hypertension
Ramos González, David. (Universitat Autònoma de Barcelona. Centre de Biotecnologia Animal i de Teràpia Gènica (CBATEG))
Catita, J. (Universidade Lusófona de Lisboa)
Ĺopez-Luppo, M. (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Valença, A. (Universitat Autònoma de Barcelona. Centre de Biotecnologia Animal i de Teràpia Gènica (CBATEG))
Bonet, Aina (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Carretero i Romay, Ana (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Navarro, M. (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Nacher García, Víctor. (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Mendez-Ferrer, S. (University of Cambridge)
Meseguer, A. (Red de Investigación Renal (REDINREN))
Casellas, Alba (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas)
Mendes-Jorge, L. (Universitat Autònoma de Barcelona. Centre de Biotecnologia Animal i de Teràpia Gènica (CBATEG))
Ruberte, J. (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)

Data:	2019
Resum:	PURPOSE. It has been suggested that arteriolar annuli localized in retinal arterioles regulate retinal blood flow acting as sphincters. Here, the morphology and protein expression profile of arteriolar annuli have been analyzed under physiologic conditions in the retina of wildtype, β-actin-Egfp, and Nestin-gfp transgenic mice. Additionally, to study the effect of hypertension, the KAP transgenic mouse has been used. METHODS. Cellular architecture has been studied using digested whole mount retinas and transmission electron microscopy. The profile of protein expression has been analyzed on paraffin sections and whole mount retinas by immunofluorescence and histochemistry. RESULTS. The ultrastructural analysis of arteriolar annuli showed a different cell population found between endothelial and muscle cells that matched most of the morphologic criteria established to define interstitial Cajal cells. The profile of protein expression of these vascular interstitial cells (VICs) was similar to that of interstitial Cajal cells and different from the endothelial and smooth muscle cells, because they expressed b-actin, nestin, and CD44, but they did not express CD31 and a-SMA or scarcely express F-actin. Furthermore, VICs share with pericytes the expression of NG2 and platelet-derived growth factor receptor beta (PDGFR-β). The high expression of Ano1 and high activity of nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase observed in VICs was diminished during hypertensive retinopathy suggesting that these cells might play a role on the motility of arteriolar annuli and that this function is altered during hypertension. CONCLUSIONS. A novel type of VICs has been described in the arteriolar annuli of mouse retina. Remarkably, these cells undergo important molecular modifications during hypertensive retinopathy and might thus be a therapeutic target against this disease.
Ajuts:	Instituto de Salud Carlos III PI12/00605 Instituto de Salud Carlos III PI16/00719 Instituto de Salud Carlos III SAF2014-59945-R
Nota:	The authors thank Veronica Melgarejo, Lorena Noya, and Angel Vazquez for technical assistance. Supported by grants from Instituto de Salud Carlos III (PI12/00605, PI16/00719, SAF2014-59945-R, and Red de Investigación Renal REDinREN 12/0021/0013), Spain; Fundação para a Ciência e a Tecnologia (SFRH/BPD/102573/2014, SFRH/BD/95330/2013), Ministerio da Educação e Ciência, Portugal; and Fondo Europeo de Desarrollo Regional (FEDER).
Nota:	Supported by grants from Instituto de Salud Carlos III (PI12/00605, PI16/00719, SAF2014-59945-R, and Red de Investigacion Renal REDinREN 12/0021/0013), Spain; Fundacao para a Ciencia e a Tecnologia (SFRH/BPD/102573/2014, SFRH/BD/95330/2013), Ministerio da Educacao e Ciencia, Portugal; and Fondo Europeo de Desarrollo Regional (FEDER).
Nota:	Altres ajuts: REDinREN/12/0021/0013
Nota:	Altres ajuts: FEDER/SFRH/BD/95330/2013
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Retina ; Arteriolar annuli ; Hypertensive retinopath
Publicat a:	Investigative Ophthalmology & Visual Science, Vol. 60 Núm. 2 (february 2019) , p. 473-487, ISSN 1552-5783

DOI: 10.1167/iovs.18-25000
PMID: 30707220

15 p, 3.0 MB

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