Interfacing neurons on carbon nanotubes covered with diamond
Seyock, Silke (Institute of Complex Systems)
Maybeck, Vanessa (Institute of Complex Systems)
Scorsone, Emmanuel (Diamond Sensors Laboratory)
Rousseau, Lionel (Université Paris Est)
Hébert, Clément (Institut Català de Nanociència i Nanotecnologia)
Lissorgues, Gaëlle (Université Paris Est)
Bergonzo, Philippe (Diamond Sensors Laboratory)
Offenhäusser, Andreas (Institute of Complex Systems)

Data:	2017
Resum:	A recently discovered material, carbon nanotubes covered with diamond (DCNTs) was tested for its suitability in bioelectronics applications. Diamond shows advantages for bioelectronics applications (wide electro chemical window and bioinertness). This study investigates the effect of electrode surface shape (flat or three dimensional) on cell growth and behavior. For comparison, flat nanocrystalline diamond substrates were used. Primary embryonic neurons were grown on top of the structures and neither incorporated the structures nor did they grow in between the single structures. The interface was closely examined using focused ion beam (FIB) and scanning electron microscopy. Of special interest was the interface between cell and substrate. 5% to 25% of the cell membrane adhered to the substrate, which fits the theoretical estimated value. While investigating the conformity of the neurons, it could be observed that the cell membrane attaches to different heights of the tips of the 3D structure. However, the aspect ratio of the structures had no effect on the cell viability. These results let us assume that not more than 25% of cell attachment is needed for the survival of a functional neuronal cell.
Ajuts:	European Commission 280433
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Cell attachments ; Cell viability ; Chemical window ; Different heights ; Electrode surfaces ; Nano-crystalline diamonds ; Neuronal cell ; Single structure
Publicat a:	RSC advances, Vol. 7, Issue 1 (2017) , p. 153-160, ISSN 2046-2069

DOI: 10.1039/c6ra20207a

8 p, 1.0 MB

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