Skin microdialysis : methods, applications and future opportunities-an EAACI position paper
Baumann, Katrine Y. (RefLab ApS, Copenhagen, Denmark)
Church, Martin K. (Charité - Universitätsmedizin Berlin)
Clough, Geraldine F. (University of Southampton)
Quist, Sven Roy (Otto-von-Guericke University)
Schmelz, Martin (Heidelberg University)
Skov, Per Stahl (RefLab ApS, Copenhagen, Denmark)
Anderson, Chris D. (Linköping University)
Tannert, Line Kring (Odense University Hospital (Dinamarca))
Giménez-Arnau, Ana M 1961- (Universitat Autònoma de Barcelona. Departament de Medicina)
Frischbutter, Stefan (Charité - Universitätsmedizin Berlin)
Scheffel, Jörg (Charité - Universitätsmedizin Berlin)
Maurer, Marcus (Charité - Universitätsmedizin Berlin)

Fecha:	2019
Resumen:	Skin microdialysis (SMD) is a versatile sampling technique that can be used to recover soluble endogenous and exogenous molecules from the extracellular compartment of human skin. Due to its minimally invasive character, SMD can be applied in both clinical and preclinical settings. Despite being available since the 1990s, the technique has still not reached its full potential use as a tool to explore pathophysiological mechanisms of allergic and inflammatory reactions in the skin. Therefore, an EAACI Task Force on SMD was formed to disseminate knowledge about the technique and its many applications. This position paper from the task force provides an overview of the current use of SMD in the investigation of the pathogenesis of chronic inflammatory skin diseases, such as atopic dermatitis, chronic urticaria, psoriasis, and in studies of cutaneous events during type 1 hypersensitivity reactions. Furthermore, this paper covers drug hypersensitivity, UVB-induced- and neurogenic inflammation, and drug penetration investigated by SMD. The aim of this paper is to encourage the use of SMD and to make the technique easily accessible by providing an overview of methodology and applications, supported by standardized operating procedures for SMD in vivo and ex vivo.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article de revisió ; Article ; Versió publicada
Materia:	Microdialysis ; Cutaneous ; Inflammation ; Interstitial ; Dermal
Publicado en:	Clinical and translational allergy, Vol. 9 (april 2019) , ISSN 2045-7022

DOI: 10.1186/s13601-019-0262-y
PMID: 31007896

13 p, 1.1 MB

El registro aparece en las colecciones:
Artículos > Artículos publicados