Web of Science: 12 cites, Scopus: 11 cites, Google Scholar: cites,
EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer
Roman-Canal, Berta (Universitat Autònoma de Barcelona)
Tarragona, Jordi (Department of Pathology and Molecular Genetics/Oncologic Pathology Group, Biomedical Research Institute of Lleida (IRBLleida), University of Lleida, CIBERONC)
Moiola, Cristian Pablo (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Gatius, Sònia (Department of Pathology and Molecular Genetics/Oncologic Pathology Group, Biomedical Research Institute of Lleida (IRBLleida), University of Lleida, CIBERONC)
Bonnin, Sarah (Centre for Genomic Regulation (CRG), The Barcelona Institute or Science and Technology)
Ruiz-Miró, Maria (Department of Pathology and Molecular Genetics/Oncologic Pathology Group, Biomedical Research Institute of Lleida (IRBLleida), University of Lleida, CIBERONC)
Sierra, José Enrique (Institut de Recerca Biomèdica)
Rufas, Maria (Institut de Recerca Biomèdica)
González, Esperanza (CIBEREHD Bizkaia Technology Park. Exosomes Laboratory and Metabolomics Platform, CIC bioGUNE)
Porcel, José M. (Arnau de Vilanova University Hospital, IRBLleida. Pleural Medicine Unit)
Gil-Moreno, Antonio (Hospital Universitari Vall d'Hebron)
Falcón-Pérez, Juan M. (IKERBASQUE, Basque Foundation for Science)
Ponomarenko, Julia (Universitat Pompeu Fabra)
Matias-Guiu, Xavier (Universitat de Lleida, Institut de Recerca Biomèdica de Lleida (IRBLleida))
Colas, Eva (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Universitat Autònoma de Barcelona

Data: 2019
Resum: Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk. In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. 210 miRNAs were significantly dysregulated (adjusted p value < 0. 05 and abs(logFC) ≥ 1). The top-10 miRNAs, which had the AUC value higher than 0. 95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p. This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC. The online version of this article (10. 1186/s12967-019-1954-8) contains supplementary material, which is available to authorized users.
Nota: Número d'acord de subvenció ISCIII/PIE15/00029
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: article ; recerca ; publishedVersion
Matèria: Colorectal cancer ; Biomarkers ; Diagnostic ; Mirnas ; Ascitic fluid ; Peritoneal lavage ; Liquid biopsy ; Extracellular vesicles ; Colon cancer
Publicat a: Journal of translational medicine, Vol. 17 (june 2019) , ISSN 1479-5876

DOI: 10.1186/s12967-019-1954-8
PMID: 31221189


14 p, 2.8 MB

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