Kisspeptin influences the reproductive axis and circulating levels of micrornas in senegalese sole
Oliveira, Catarina (University of Algarve. Center of Marine Sciences)
Fatsini, Elvira (University of Algarve. Center of Marine Sciences)
Fernández, Ignacio (Instituto Tecnológico Agrario de Castilla y León)
Anjos, Catarina (University of Algarve. Center of Marine Sciences)
Chauvigné, François (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Cerdà, Joan (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Mjelle, Robin (Nord University. Faculty of Bioscience and Aquaculture)
Fernandes, Jorge (Nord University. Faculty of Bioscience and Aquaculture)
Cabrita, Elsa (University of Algarve. Center of Marine Sciences)

Fecha:	2020
Resumen:	Kisspeptin regulates puberty and reproduction onset, acting upstream of the brain-pituitary-gonad (HPG) axis. This study aimed to test a kisspeptin-based hormonal therapy on cultured Senegalese sole (G1) breeders, known to have reproductive dysfunctions. A single intramuscular injection of KISS2-10 decapeptide (250 µg/kg) was tested in females and males during the reproductive season, and gonad maturation, sperm motility, plasma levels of gonadotropins (Fsh and Lh) and sex steroids (11-ketotestosterone, testosterone and estradiol), as well as changes in small non-coding RNAs (sncRNAs) in plasma, were investigated. Fsh, Lh, and testosterone levels increased after kisspeptin injection in both sexes, while sperm analysis did not show differences between groups. Let7e, miR-199a-3p and miR-100-5p were differentially expressed in females, while miR-1-3p miRNA was up-regulated in kisspeptin-treated males. In silico prediction of mRNAs targeted by miRNAs revealed that kisspeptin treatment might affect paracellular transporters, regulate structural and functional polarity of cells, neural networks and intracellular trafficking in Senegalese sole females; also, DNA methylation and sphingolipid metabolism might be altered in kisspeptin-treated males. Results demonstrated that kisspeptin stimulated gonadotropin and testosterone secretion in both sexes and induced an unanticipated alteration of plasma miRNAs, opening new research venues to understand how this neuropeptide impacts in fish HPG axis.
Ayudas:	Ministerio de Ciencia e Innovación PID2019-103868RB-I00 European Commission 683210 Ministerio de Ciencia e Innovación RYC-2018-025337-I Ministerio de Ciencia e Innovación RYC--2015-17103
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Solea senegalensis ; G1 breeders ; Hormonal treatment ; KISS2 ; Gonadotropins ; Sex steroids
Publicado en:	International journal of molecular sciences, Vol. 21, Issue 23 (December 2020) , art. 9051, ISSN 1422-0067

DOI: 10.3390/ijms21239051
PMID: 33260781

21 p, 3.0 MB

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