Fine mapping and identification of candidate genes for the peach powdery mildew resistance gene Vr3
Marimon, Neus (Centre de Recerca en Agrigenòmica)
Luque, Jordi (Institut de Recerca i Tecnologia Agroalimentàries)
Arús i Gorina, Pere (Centre de Recerca en Agrigenòmica)
Eduardo, Iban (Centre de Recerca en Agrigenòmica)

Date:	2020
Abstract:	Powdery mildew is one of the major diseases of peach (Prunus persica), caused by the ascomycete Podosphaera pannosa. Currently, it is controlled through calendar-based fungicide treatments starting at petal fall, but an alternative is to develop resistant peach varieties. Previous studies mapped a resistance gene (Vr3) in interspecific populations between almond ('Texas') and peach ('Earlygold'). To obtain molecular markers highly linked to Vr3 and to reduce the number of candidate genes, we fine-mapped Vr3 to a genomic region of 270 kb with 27 annotated genes. To find evidence supporting one of these positional candidate genes as being responsible of Vr3, we analyzed the polymorphisms of the resequences of both parents and used near-isogenic lines (NILs) for expression analysis of the positional candidate genes in symptomatic or asymptomatic leaves. Genes differentially expressed between resistant and susceptible individuals were annotated as a Disease Resistance Protein RGA2 (Prupe2G111700) or an Eceriferum 1 protein involved in epicuticular wax biosynthesis (Prupe2G112800). Only Prupe2G111700 contained a variant predicted to have a disruptive effect on the encoded protein, and was overexpressed in both heterozygous and homozygous individuals containing the Vr3 almond allele, compared with susceptible individuals. This information was also useful to identify and validate molecular markers tightly linked and flanking Vr3. In addition, the NILs used in this work will facilitate the introgression of this gene into peach elite materials, alone or pyramided with other known resistance genes such as peach powdery mildew resistance gene Vr2.
Grants:	Ministerio de Economía y Competitividad RTA2015-00050-00-00 Ministerio de Economía y Competitividad RTA2013-00004-C03-01 Ministerio de Economía y Competitividad AGL2015-68329-R Ministerio de Economía y Competitividad SEV-2015-0533
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Horticulture research, Vol. 7 (November 2020) , art. 175, ISSN 2052-7276

DOI: 10.1038/s41438-020-00396-9
PMID: 33328431

9 p, 467.5 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles