Influence of a polymer membrane on the electrochemical determination of insulin in nanomodified screen printed carbon electrodes
Šišoláková, Ivana (University of P. J. Šafárik in Košice. Department of Physical Chemistry)
Hovancová, Jana (University of P. J. Šafárik in Košice. Department of Physical Chemistry)
Oriňaková, Renáta (University of P. J. Šafárik in Košice. Department of Physical Chemistry)
Oriňak, Andrej (University of P. J. Šafárik in Košice. Department of Physical Chemistry)
Trnková, Libuše (Masaryk University. Department of Chemistry)
Rueda García, Daniel (Institut de Ciència de Materials de Barcelona)
Radoňák, Jozef (University of P. J. Šafárik in Košice. Faculty of Medicine)

Fecha:	2019
Resumen:	Diabetes mellitus can be considered one of the most widespread diseases globally. Hence, the diabetes research is currently focused on developing an effective, low-cost sensor having high stability and suitable analytical characteristics. Screen printed carbon electrodes (SPCEs) embody ideal candidates for insulin determination due to the small area of the working electrode eliminating the solution volume required for the given purpose. Modification of SPCEs by using nanoparticles resulted in an increase of the working electrode surface area and formation of a higher number of active species. The aim of this paper is to examine the impact of a chitosan membrane on the electrochemical determination of insulin on NiO nanoparticles (NiONPs) and multi-walled nanotube (MWCNTs) modified SPCE (NiONPs/MWCNTs/SPCE). This study is primarily conceived to compare the analytical characteristics and stability of NiONPs/chitosan-MWCNTs/SPCE and NiONPs/MWCNTs/SPCE. An electrode modified with chitosan displays a wider linear range, one of 0. 25 μM - 5 μM (R² 0. 997); a lower limit of detection, 94 nM; a high sensitivity (0. 021 μA/μM) and better stability than that of an electrode without chitosan. According to these characteristics, the polymer is considered a necessary compound of the electrochemical insulin sensor, improving the sensor's analytical characteristics.
Nota:	Ajuts: the research was supported by the projects VEGA 1/0074/17 of the Slovak Scientific Grant Agency, APVV-16-0029 of the Slovak Research and Development Agency, and VVGS-PF-2018-795 and VVGS-PF-2018-794 of Pavol Jozef Šafárik University in Košice.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió sotmesa a revisió
Materia:	Insulin determination ; Screen printed carbon electrodes ; Polymer membrane ; Nickel oxide nanoparticles ; Chitosan
Publicado en:	Bioelectrochemistry, Vol. 130 (Dec.2019) , art. 107326, ISSN 1878-562X

DOI: 10.1016/j.bioelechem.2019.06.011
PMID: 31295699

Preprint 26 p, 1.7 MB

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