Incorporation of 5-bromo-2'-deoxyuridine into DNA and proliferative behavior of cerebellar neuroblasts : all that glitters is not gold
Martí-Clúa, Joaquín (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Universitat Autònoma de Barcelona. Institut de Neurociències

Data:	2021
Resum:	The synthetic halogenated pyrimidine analog, 5-bromo-2-deoxyuridine (BrdU), is a marker of DNA synthesis. This exogenous nucleoside has generated important insights into the cellular mechanisms of the central nervous system development in a variety of animals including insects, birds, and mammals. Despite this, the detrimental effects of the incorporation of BrdU into DNA on proliferation and viability of different types of cells has been frequently neglected. This review will summarize and present the effects of a pulse of BrdU, at doses ranging from 25 to 300 µg/g, or repeated injections. The latter, following the method of the progressively delayed labeling comprehensive procedure. The prenatal and perinatal development of the cerebellum are studied. These current data have implications for the interpretation of the results obtained by this marker as an index of the generation, migration, and settled pattern of neurons in the developing central nervous system. Caution should be exercised when interpreting the results obtained using BrdU. This is particularly important when high or repeated doses of this agent are injected. I hope that this review sheds light on the effects of this toxic maker. It may be used as a reference for toxicologists and neurobiologists given the broad use of 5-bromo-2-deoxyuridine to label dividing cells.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Prenatal life ; Perinatal life ; 5-bromo-2'-deoxyuridine ; Cell cycle ; Cerebellar neuroepithelium ; External granular layer ; Neurogenetic timetables ; Neurogenetic gradients ; Apoptosis
Publicat a:	Cells, Vol. 10, Issue 6 (June 2021) , art. 1453, ISSN 2073-4409

DOI: 10.3390/cells10061453
PMID: 34200598

15 p, 2.8 MB

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