Differential distribution of the wlaN and cgtB genes, associated with Guillain-Barré syndrome, in Campylobacter jejuni isolates from humans, broiler chickens, and wild birds
Guirado Frias, Pedro León (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Paytubi Casabona, Sònia (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Miró, Elisenda (Institut d'Investigació Biomèdica Sant Pau)
Iglesias Torrens, Yaidelys (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Navarro Risueño, Ferran (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Cerdà-Cuéllar, Marta (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Stephan-Otto Attolini, Camille (Institut de Recerca Biomèdica)
Balsalobre Parra, Carlos (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Madrid Xufré, Cristina (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)

Date:	2020
Abstract:	Campylobacter jejuni causes campylobacteriosis, a bacterial gastroenteritis with high incidence worldwide. Moreover, C. jejuni infection can trigger the polyneuropathic disorder denominated Guillain-Barré syndrome (GBS). The C. jejuni strains that can elicit GBS carry either wlaN or cgtB, coding both genes for a β-1,3-galactosyltransferase enzyme that is required for the production of sialylated lipooligosaccharide (LOS SIAL). We described a differential prevalence of the genes wlaN and cgtB in C. jejuni isolates from three different ecological niches: humans, broiler chickens, and wild birds. The distribution of both genes, which is similar between broiler chicken and human isolates and distinct when compared to the wild bird isolates, suggests a host-dependent distribution. Moreover, the prevalence of the wlaN and cgtB genes seems to be restricted to some clonal complexes. Gene sequencing identified the presence of new variants of the G- homopolymeric tract within the wlaN gene. Furthermore, we detected two variants of a G rich region within the cgtB gene, suggesting that, similarly to wlaN, the G-tract in the cgtB gene mediates the phase variation control of cgtB expression. Caco-2 cell invasion assays indicate that there is no evident correlation between the production of LOS SIAL and the ability to invade eukaryotic cells.
Grants:	Agencia Estatal de Investigación PGC2018-096958-B-I00 Ministerio de Economía y Competitividad AGL2013-45339R Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-499
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Campylobacter ; Lipooligosaccharide ; Wlan ; Cgtb ; Guillain-Barré syndrome
Published in:	Microorganisms, Vol. 8, issue 3 (March 2020) , art. 325, ISSN 2076-2607

DOI: 10.3390/microorganisms8030325
PMID: 32110976

11 p, 1.7 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles