Chitinase 3-like 1 is neurotoxic in primary cultured neurons
Matute-Blanch, Clara (Hospital Universitari Vall d'Hebron)
Calvo-Barreiro, Laura (Hospital Universitari Vall d'Hebron)
Carballo-Carbajal, Iria (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Gonzalo, Ricardo (Hospital Universitari Vall d'Hebron)
Sánchez, Alex (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Vila Bover, Miquel (Institució Catalana de Recerca i Estudis Avançats)
Montalban, Xavier (St. Michael's Hospital, University of Toronto)
Comabella, Manuel (Hospital Universitari Vall d'Hebron)

Data:	2020
Resum:	Chitinase 3-like 1 (CHI3L1) is known to play a role as prognostic biomarker in the early stages of multiple sclerosis (MS), and patients with high cerebrospinal fluid CHI3L1 levels have an increased risk for the development of neurological disability. Here, we investigated its potential neurotoxic effect by adding recombinant CHI3L1 in vitro to primary cultures of mouse cortical neurons and evaluating both neuronal functionality and survival by immunofluorescence. CHI3L1 induced a significant neurite length retraction after 24 and 48 hours of exposure and significantly reduced neuronal survival at 48 hours. The cytotoxic effect of CHI3L1 was neuron-specific and was not observed in mouse immune or other central nervous system cells. These results point to a selective neurotoxic effect of CHI3L1 in vitro and suggest a potential role of CHI3L1 as therapeutic target in MS patients.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Diagnostic markers ; Predictive markers ; Prognostic markers ; Cell death in the nervous system
Publicat a:	Scientific reports, Vol. 10 (april 2020) , ISSN 2045-2322

DOI: 10.1038/s41598-020-64093-2
PMID: 32346016

6 p, 2.1 MB

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