Unveiling the effect of dietary essential oils supplementation in Sparus aurata gills and its efficiency against the infestation by Sparicotyle chrysophrii
Firmino, Joana P (Universitat Autònoma de Barcelona)
Vallejos Vidal, Eva Carolina (Universidad de Santiago de Chile. Centro de Biotecnología Acuícola)
Sarasquete, Carmen (Universidad de Cádiz. Instituto de Ciencias Marinas de Andalucía)
Ortiz-Delgado, Juan B. (Universidad de Cádiz. Instituto de Ciencias Marinas de Andalucía)
Balasch Alemany, Joan Carles (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Tort Bardolet, Lluís (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Estevez, Alicia (Institut de Recerca i Tecnologia Agroalimentàries)
Reyes-López, Felipe E. (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Gisbert, Enric (Institut de Recerca i Tecnologia Agroalimentàries)

Data:	2020
Resum:	A microencapsulated feed additive composed by garlic, carvacrol and thymol essential oils (EOs) was evaluated regarding its protective effect in gills parasitized by Sparicotyle chrysophrii in Sparus aurata. A nutritional trial (65 days) followed by a cohabitation challenge with parasitized fish (39 days) were performed. Transcriptomic analysis by microarrays of gills of fish fed the EOs diet showed an up-regulation of genes related to biogenesis, vesicular transport and exocytosis, leukocyte-mediated immunity, oxidation-reduction and overall metabolism processes. The functional network obtained indicates a tissue-specific pro-inflammatory immune response arbitrated by degranulating acidophilic granulocytes, sustained by antioxidant and anti-inflammatory responses. The histochemical study of gills also showed an increase of carboxylate glycoproteins containing sialic acid in mucous and epithelial cells of fish fed the EOs diet, suggesting a mucosal defence mechanism through the modulation of mucin secretions. The outcomes of the in vivo challenge supported the transcriptomic results obtained from the nutritional trial, where a significant reduction of 78% in the abundance of S. chrysophrii total parasitation and a decrease in the prevalence of most parasitic developmental stages evaluated were observed in fish fed the EOs diet. These results suggest that the microencapsulation of garlic, carvacrol and thymol EOs could be considered an effective natural dietary strategy with antiparasitic properties against the ectoparasite S. chrysophrii.
Nota:	Altres ajuts: Industrial PhD program of Generalitat de Catalunya (Doctorats Industrials) and TECNOVIT-FARMFAES S.L. (No. 2017 DI 017)
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Immunology ; Infection ; Innate immunity ; Molecular biology ; Transcriptomics ; Pathogens ; Gene expression analysis ; Microarray analysis
Publicat a:	Scientific reports, Vol. 10 (October 2020) , art. 17764, ISSN 2045-2322

DOI: 10.1038/s41598-020-74625-5
PMID: 33082387

23 p, 22.4 MB

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