Assessment of gold nanoparticles on human peripheral blood cells by metabolic profiling with 1 H-NMR spectroscopy, a novel translational approach on a patient-specific basis
Palomino-Schätzlein, Martina (Centro de Investigación Príncipe Felipe (València))
García, Hermenegildo (Instituto Universitario de Tecnología Química CSIC-UPV (València))
Gutiérrez-Carcedo, Patricia (Hospital Universitari Vall d'Hebron)
Pineda-Lucena, Antonio (Instituto de Investigación Sanitaria La Fe)
Herance, José Raul (Hospital Universitari Vall d'Hebron)
Universitat Autònoma de Barcelona

Data:	2017
Resum:	Human peripheral blood cells are relevant ex vivo models for characterizing diseases and evaluating the pharmacological effects of therapeutic interventions, as they provide a close reflection of an individual pathophysiological state. In this work, a new approach to evaluate the impact of nanoparticles on the three main fractions of human peripheral blood cells by nuclear magnetic resonance spectroscopy is shown. Thus, a comprehensive protocol has been set-up including the separation of blood cells, their in vitro treatment with nanoparticles and the extraction and characterization of metabolites by nuclear magnetic resonance. This method was applied to assess the effect of gold nanoparticles, either coated with chitosan or supported on ceria, on peripheral blood cells from healthy individuals. A clear antioxidant effect was observed for chitosan-coated gold nanoparticles by a significant increase in reduced glutathione, that was much less pronounced for gold-cerium nanoparticles. In addition, the analysis revealed significant alterations of several other pathways, which were stronger for gold-cerium nanoparticles. These results are in accordance with the toxicological data previously reported for these materials, confirming the value of the current methodology.
Ajuts:	Ministerio de Economía y Competitividad CP13/00252 Ministerio de Economía y Competitividad PI16/02064 Ministerio de Economía y Competitividad SAF2014-53977-R
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	PloS one, Vol. 12 (august 2017) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0182985
PMID: 28793337

19 p, 5.8 MB

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