DAX-1 Expression in Pediatric Rhabdomyosarcomas : Another Immunohistochemical Marker Useful in the Diagnosis of Translocation Positive Alveolar Rhabdomyosarcoma
Virgone, Calogero (University Hospital of Padova (Pàdua, Itàlia))
Lalli, Enzo (Université de Nice-Sophia Antipolis)
Bisogno, Gianni (University Hospital of Padova (Pàdua, Itàlia))
Lazzari, Elena (San Bortolo Hospital, Vicenza)
Roma, Josep (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Zin, Angelica (Istituto della Ricerca Pediatrica "Città della Speranza")
Poli, Elena (Istituto della Ricerca Pediatrica "Città della Speranza")
Cecchetto, Giovanni (University Hospital of Padova (Pàdua, Itàlia))
Dall'Igna, Patrizia (University Hospital of Padova (Pàdua, Itàlia))
Alaggio, Rita (University of Padua)
Universitat Autònoma de Barcelona

Data:	2015
Resum:	The aim of this study was to investigate the expression of DAX-1 in a series of pediatric rhabdomyosarcomas (RMS) with known translocation and compare it to Ap2β, known to be selectively expressed in ARMS. We revised a series of 71 alveolar rhabdomyosarcomas (ARMS), enrolled in the Italian Protocols RMS 79 and 96, and 23 embryonal rhabdomyosarcomas (ERMS) as controls. Before investigating Ap2β and DAX-1, ARMS were reviewed and reclassified as 48 ARMS and 23 non-ARMS. Translocation positive ARMS showed a characteristic Ap2β/DAX-1+ staining pattern in 78% of cases, while 76% of classic ERMS were negative for both. Ap2β alone was positive in 3. 9% of RMS lacking translocation, whereas DAX-1 alone was positive in 25. 4%. Conversely, 9% and 6% of translocation positive ARMS were positive only for DAX-1 or Ap2β, respectively. The 23 non-ARMS shared the same phenotype as ERMS but had a higher frequency of DAX-1 expression. DAX-1 is less specific than Ap2β, however it is a sensitive marker for translocation positive ARMS and can be helpful in their diagnosis if used in combination with Ap2β.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	PloS one, Vol. 10 (july 2015) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0133019
PMID: 26168243

12 p, 2.0 MB

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