Skin Multi-Omics-Based Interactome Analysis : Integrating the Tissue and Mucus Exuded Layer for a Comprehensive Understanding of the Teleost Mucosa Functionality as Model of Study
Reyes Lopez, Felipe Esteban (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Ibarz, Antoni (Universitat de Barcelona. Departament de Biologia Cel·lular, Fisiologia i Immunologia)
Ordóñez Grande, Borja (Universitat de Barcelona. Departament de Biologia Cel·lular, Fisiologia i Immunologia)
Vallejos Vidal, Eva Carolina (Universidad de Santiago de Chile. Centro de Biotecnología Acuícola)
Andree, Karl B. (Institut de Recerca i Tecnologia Agroalimentàries)
Balasch Alemany, Joan Carles (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Fernández-Alacid, Laura (Universitat de Barcelona. Departament de Biologia Cel·lular, Fisiologia i Immunologia)
Sanahuja, Ignasi (Universitat de Barcelona. Departament de Biologia Cel·lular, Fisiologia i Immunologia)
Sánchez-Nuño, Sergio (Universitat de Barcelona. Departament de Biologia Cel·lular, Fisiologia i Immunologia)
Firmino, Joana P. (Universitat Autònoma de Barcelona)
Pavez, Leonardo (Universidad de las Américas. Instituto de Ciencias Naturales)
Polo, Javier (APC Europe SL)
Tort Bardolet, Lluís (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Gisbert, Enric (Institut de Recerca i Tecnologia Agroalimentàries)

Data:	2021
Resum:	From a general structural perspective, a mucosal tissue is constituted by two main matrices: the tissue and the secreted mucus. Jointly, they fulfill a wide range of functions including the protection of the epithelial layer. In this study, we simultaneously analyzed the epithelial tissue and the secreted mucus response using a holistic interactome-based multi-omics approach. The effect of the gilthead sea bream (Sparus aurata) skin mucosa to a dietary inclusion of spray-dried porcine plasma (SDPP) was evaluated. The epithelial skin microarrays-based transcriptome data showed 194 differentially expressed genes, meanwhile the exuded mucus proteome analysis 35 differentially synthesized proteins. Separately, the skin transcripteractome revealed an expression profile that favored biological mechanisms associated to gene expression, biogenesis, vesicle function, protein transport and localization to the membrane. Mucus proteome showed an enhanced protective role with putatively higher antioxidant and antimicrobial properties. The integrated skin mucosa multi-interactome analysis evidenced the interrelationship and synergy between the metabolism and the exuded mucus functions improving specifically the tissue development, innate defenses, and environment recognition. Histologically, the skin increased in thickness and in number of mucous cells. A positive impact on animal performance, growth and feed efficiency was also registered. Collectively, the results suggest an intimate crosstalk between skin tissue and its exuded mucus in response to the nutritional stimulus (SDPP supplementation) that favors the stimulation of cell protein turnover and the activation of the exudation machinery in the skin mucosa. Thus, the multi-omics-based interactome analysis provides a comprehensive understanding of the biological context of response that takes place in a mucosal tissue. In perspective, this strategy is applicable for evaluating the effect of any experimental variable on any mucosal tissue functionality, including the benefits this assessment may provide on the study of the mammalian mucosa.
Ajuts:	Ministerio de Economía y Competitividad AGL2014-51839-C5-5-R Ministerio de Economía y Competitividad AGL2015-70637-R European Commission 727315
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Microarrays ; Multi-omics analyses ; Functional networks ; Integrative analysis ; Immune response ; Histology ; Skin mucosa ; Functional diet
Publicat a:	Frontiers in immunology, Vol. 11 (February 2021) , art. 613824, ISSN 1664-3224

DOI: 10.3389/fimmu.2020.613824
PMID: 33613538

20 p, 4.3 MB

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