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Pàgina inicial > Articles > Articles publicats > Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco |
Data: | 2021 |
Resum: | In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase-glutamate synthase pathway; specifically tobacco plants overexpressing Trx m displayed increased activity and stability of glutamine synthetase. Moreover, higher photorespiration and nitrate accumulation were observed in these plants relative to untransformed control plants, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, our results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts. |
Ajuts: | Ministerio de Ciencia e Innovación AGL2016-79868 Ministerio de Educación, Cultura y Deporte FPU13/01675 European Commission 753301 Agencia Estatal de Investigación RYC2019-027244-I |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Matèria: | Carbon metabolism ; Chloroplast ; Glutamine synthetase ; GS-GOGAT pathway ; Nitrogen metabolism ; Photorespiration ; Thioredoxin |
Publicat a: | Journal of experimental botany, Vol. 72, Issue 13 (May 2021) , p. 4949-4964, ISSN 1460-2431 |
16 p, 1.8 MB |