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| Pàgina inicial > Articles > Articles publicats > Quantitative GC-TCD Measurements of Major Flatus Components : |
| Data: | 2022 |
| Resum: | The impact of diet and digestive disorders in flatus composition remains largely unexplored. This is partially due to the lack of standardized sampling collection methods, and the easy atmospheric contamination. This paper describes a method to quantitatively determine the major gases in flatus and their application in a nutritional intervention. We describe how to direct sample flatus into Tedlar bags, and simultaneous analysis by gas chromatography-thermal conductivity detection (GC-TCD). Results are analyzed by univariate hypothesis testing and by multilevel principal component analysis. The reported methodology allows simultaneous determination of the five major gases with root mean measurement errors of 0. 8% for oxygen (O), 0. 9% for nitrogen (N), 0. 14% for carbon dioxide (CO), 0. 11% for methane (CH), and 0. 26% for hydrogen (H). The atmospheric contamination was limited to 0. 86 (95% CI: [0. 7-1. 0])% for oxygen and 3. 4 (95% CI: [1. 4-5. 3])% for nitrogen. As an illustration, the method has been successfully applied to measure the response to a nutritional intervention in a reduced crossover study in healthy subjects. |
| Ajuts: | Agencia Estatal de Investigación RTI2018-098577-B-C21 Agencia Estatal de Investigación RTI2018-098577-B-C22 European Commission 712754 Ministerio de Economía y Competitividad 2016-76648-R |
| Nota: | Altres ajuts: Departament d'Universitats, Recerca i Societat de la Informació de la Generalitat de Catalunya (expedient 2017 SGR 1721) |
| Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
| Llengua: | Anglès |
| Document: | Article ; recerca ; Versió publicada |
| Matèria: | Major flatus gas components ; Diet effect on flatus ; Multilevel principal component analysis ; Rectal gas collection |
| Publicat a: | Sensors (Basel, Switzerland), Vol. 22 (january 2022) , ISSN 1424-8220 |
14 p, 1.3 MB |