Evaluation of the Probiotic In Vitro Potential of Lactic Acid-Producing Bacteria from Canine Vagina : possible Role in Vaginal Health
Morales, Brian (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Spadetto, Livia (Universidad de Murcia. Grupo de Investigación en Toxicología)
Calvo Torras, Ma. de los Ángeles (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Yeste Oliveras, Marc (Universitat de Girona. Departament de Biologia)
Arosemena, Leonardo (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Rigau i Mas, Teresa (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Rivera del Álamo, Maria Montserrat (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Data:	2022
Resum:	Lactic acid-producing bacteria (LAB) are being widely studied due to their probiotic potential. The aim of the present study was to determine and identify the presence of LAB from canine vaginal samples, as well as to evaluate their probiotic in vitro potential. Ninety-four bitches were included in the study. Vaginal samples were obtained by means of a sterile swab and streaked on Man Rogosa Sharpe agar plates. A total of 100 LAB strains were obtained and submitted to Gram stains and basic biochemical tests, which included catalase, oxidase and haemolysis tests. Thirteen strains belonging to the genera Lactobacillus (n = 10), Lactococcus (n = 2) and Pediococcus (n = 1) were selected as potential probiotics and further subjected to evaluation of resistance to gastrointestinal conditions (pH, lysozyme, bile salts and hydrogen peroxide) and safety and efficacy in vitro (resistance to antibiotics and antimicrobial capacity). Only three strains, one Lactobacillus lactis and two Lactobacillus plantarum, accomplished the requirements for being considered as potential in vitro probiotics.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; Versió publicada
Matèria:	Canine vagina ; Probiotic ; Lactobacillus spp ; Gastrointestinal conditions ; Antimicrobial activity
Publicat a:	Animals, Vol. 12 Núm. 6 (2022) , p. 796, ISSN 2076-2615

DOI: 10.3390/ani12060796
PMID: 35327193

12 p, 303.6 KB

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