Fluorescence Imaging Characterization of the Separation Process in a Monolithic Microfluidic Free-Flow Electrophoresis Device Fabricated Using Low-Temperature Co-Fired Ceramics
Couceiro, Pedro (Universitat Autònoma de Barcelona. Departament de Química)
Alonso Chamarro, Julián (Universitat Autònoma de Barcelona. Departament de Química)

Data:	2022
Resum:	A monolithic microfluidic free-flow electrophoresis device, fabricated using low-temperature co-fired ceramic technology, is presented. The device integrates gold electrodes and a 20 µm thick transparent ceramic optical window, suitable for fluorescence imaging, into a multilevel microfluidic chamber design. The microfluidic chamber consists of a 60 µm deep separation chamber and two, 50 µm deep electrode chambers separated by 10 µm deep side channel arrays. Fluorescence imaging was used for in-chip, spatial-temporal characterization of local pH variations in separation conditions as well as to characterize the separation process. The device allowed baseline resolution separation of a sample mixture of Fluorescein, Rhodamine 6G, and 4-Methylumbelliferone at pH 7. 0, in only 6 s, using 378 V. s/cm. The results demonstrate the possibility of studying a chemical process using fluorescence imaging within the traditional fields of low-temperature co-fired ceramics technology, such as high-electrical-field applications, while using a simple fabrication procedure suitable for low-cost mass production.
Ajuts:	Agencia Estatal de Investigación PID2020-117216RB-I00
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Microfluidics ; Free-flow electrophoresis ; Fluorescence imaging ; Spatial-temporal pH characterization ; Low-temperature co-fired ceramics
Publicat a:	Micromachines, Vol. 13, Issue 7 (June 2022) , art. 1023, ISSN 2072-666X

DOI: 10.3390/mi13071023
PMID: 35888840

13 p, 1.1 MB

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