Rat age-related benign prostate hyperplasia is concomitant with an increase in the secretion of low ramified α-glycosydic polysaccharides
Trujillo-Rojas, Lina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Fernández Novell, Josep M.. (Universitat de Barcelona. Departament de Bioquímica i Biologia Molecular)
Blanco Prieto, Olga (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Martí-Garcia, Bernat (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Rigau i Mas, Teresa (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Rivera del Álamo, Maria Montserrat (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Data:	2022
Resum:	This work analysed the expression of prostate polysaccharides in rats with age-related benign prostatic hyperplasia (BPH) for a better understanding of the possible relationship between prostate polysaccharides secretion and BPH onset. For this, prostatic glands from 1 month-old, 3 months-old, 6 months-old and 12 months-old Sprague-Dawley rats were processed in order to identify their overall polysaccharide content. Additionally, serum testosterone was also determined. One-month old rats showed significantly (P < 0. 05) lower testosterone levels (0. 77 ng/mL±0. 12 ng/mL) compared with the other groups, which showed no significant difference among them. PAS staining showed positive polysaccharides markings in both the prostatic lumen and inside of luminal prostatic cells in all groups. Semiquantitative analysis of intraluminal PAS showed that one month-old rats had significantly (P < 0. 005) lower PAS intensity when compared with all other groups (100. 0 ± 0. 5, arbitrary units vs. 107. 3 ± 0. 6, arbitrary units in 3 months-old ones), whereas 12 months-old ones showed significantly (P < 0. 005) higher values when compared with all other groups (133. 6 ± 3. 5, arbitrary units in 12 months-old rats vs. 108. 6 ± 1. 4, arbitrary units in 6 months-old ones). The PAS + content practically disappeared when tissues were pre-incubated with either α-amylase or amyloglucosidase, regardless of a previous incubation with proteinase K. Incubation of prostate extracts from 12 months-old rats for 2 h with α-amylase yielded a significantly higher amount of free glucose (1. 47 nmol/mg protein±0. 23 nmol/mg protein vs. 0. 32 nmol/mg protein±0. 01 nmol/mg protein in untreated extracts). Similar results were obtained when extracts were pre-incubated with amyloglucosidase. Contrarily, pre-incubation with N-glycosidase induced a significantly (P < 0. 05), much lower increase of free glucose. Pre-treatment with proteinase K did not significantly modify these results, which indicate that BPH is related to an increase in the secretion of low ramified ductal α-glycosydic polysaccharides that were not protected against lysis by any type of protein protective core. These changes seem to not be related with concomitant variations in serum testosterone levels.
Nota:	Altres ajuts: acords transformatius de la UAB
Nota:	We would like to thank Dr. Manel Bosch (Advanced Optical Microscopy Facilities, University of Barcelona) for his invaluable advice and collaboration to obtain microscope images of both Haematoxylin-Eosin-stained samples and PAS ones. No disclosure was applicable on this work. Likewise, there is no conflict of interest related with this manuscript. Furthermore, no specific Funding was afforded for this work.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Prostate hyperplasia ; Polysaccharide content
Publicat a:	Theriogenology, Vol. 189 (September 2022) , p. 150-157, ISSN 0093-691X

DOI: 10.1016/j.theriogenology.2022.06.011
PMID: 35760026

8 p, 1.7 MB

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