Biochemistry strategies for label-free optical sensor biofunctionalization : advances towards real applicability
Soler Aznar, Maria (Institut Català de Nanociència i Nanotecnologia)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)

Data:	2021
Resum:	Label-free biosensors, and especially those based on optical transducers like plasmonic or silicon photonic systems, have positioned themselves as potential alternatives for rapid and highly sensitive clinical diagnostics, on-site environmental monitoring, and for quality control in foods or other industrial applications, among others. However, most of the biosensor technology has not yet been transferred and implemented in commercial products. Among the several causes behind that, a major challenge is the lack of standardized protocols for sensor biofunctionalization. In this review, we summarize the most common methodologies for sensor surface chemical modification and bioreceptor immobilization, discussing their advantages and limitations in terms of analytical sensitivity and selectivity, reproducibility, and versatility. Special focus is placed on the suggestions of innovative strategies towards antifouling and biomimetic functional coatings to boost the applicability and reliability of optical biosensors in clinics and biomedicine. Finally, a brief overview of research directions in the area of device integration, automation, and multiplexing will give a glimpse of the future perspectives for label-free optical biosensors.
Ajuts:	Agencia Estatal de Investigación PGC-2018-099870 Ministerio de Ciencia e Innovación SEV-2017-0706
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Surface plasmon resonance ; Silicon photonics ; Antibody immobilization ; Biochemical cross-linking ; Antifouling coating ; Lipid membrane
Publicat a:	Analytical and bioanalytical chemistry, Vol. 414, Issue 18 (November 2021) , p. 5071-5085, ISSN 1618-2650

DOI: 10.1007/s00216-021-03751-4
PMID: 34735605

15 p, 2.7 MB

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