The high plasticity of nonpathogenic Mycobacterium brumae induces rapid changes in its lipid profile during pellicle maturation : The potential of this bacterium as a versatile cell factory for lipid compounds of therapeutic interest
Campo Pérez, Víctor (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Guallar Garrido, Sandra (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Luquin Fernandez, Marina de la Milagrosa (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Sánchez Chardi, Alejandro (Universitat Autònoma de Barcelona. Servei de Microscòpia)
Julián Gómez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data:	2022
Resum:	The immunomodulatory potential of mycobacteria to be used for therapeutic purposes varies by species and culture conditions and is closely related to mycobacterial lipid composition. Although the lipids present in the mycobacterial cell wall are relevant, lipids are mainly stored in intracellular lipid inclusions (ILIs), which have emerged as a crucial structure in understanding mycobacteria-host interaction. Little is known about ILI ultrastructure, production, and composition in nonpathogenic species. In this study, we compared the lipid profiles of the nonpathogenic immunomodulatory agent Mycobacterium brumae during pellicle maturation under different culture conditions with qualitative and quantitative approaches by using high-resolution imaging and biochemical and composition analyses to understand ILI dynamics. The results showed wax esters, mainly in early stages of development, and acylglycerols in mature ILI composition, revealing changes in dynamics, amount, and morphometry, depending on pellicle maturation and the culture media used. Low-glycerol cultures induced ILIs with lower molecular weights which were smaller in size in comparison with the ILIs produced in glycerol-enriched media. The data also indicate the simple metabolic plasticity of lipid synthesis in M. brumae, as well as its high versatility in generating different lipid profiles. These findings provide an interesting way to enhance the production of key lipid structures via the simple modulation of cell culture conditions.
Ajuts:	Agencia Estatal de Investigación RTI2018-098777-B-I00 Agencia Estatal de Investigación PID2021-122331OB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-229
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Cell wall ; Electron microscopy ; Lipid inclusions ; Intrabacterial ; Mycobacterium ; Ultrastructural imaging
Publicat a:	International journal of molecular sciences, Vol. 23, Issue 21 Special Issue "Microbial Lipids: Production, Characterization and Applications" (November 2022) , art. 13609, ISSN 1422-0067

DOI: 10.3390/ijms232113609
PMID: 36362396

14 p, 5.7 MB

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