Semen Modulates Cell Proliferation and Differentiation-Related Transcripts in the Pig Peri-Ovulatory Endometrium
Gardela Santacruz, Jaume (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Ruiz Conca, Mateo (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Wright, Dominic (Linköping University. Department of Physics, Chemistry and Biology)
López Béjar, Manel (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Martinez, Cristina A (Linköping University. Department of Biomedical & Clinical Sciences)
Rodriguez-Martinez, Heriberto (Linköping University. Department of Biomedical & Clinical Sciences)
Álvarez-Rodríguez, Manuel (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)

Fecha:	2022
Resumen:	Homeostasis of the uterus after mating is crucial for the subsequent reproductive events. The post-mating inflammatory response is restricted to the uterus, but semen also modulates the expression of other genes involved in regulation along the female reproductive tract, including the oviduct. This study aims to determine if several ejaculate fractions of the pig may modulate cell proliferation and differentiation-related transcripts in different sections of the peri-ovulatory sow reproductive tract. Our data demonstrate that most of the mRNA expression changes of the 144 transcripts tested were induced by mating. Additionally, spermatozoa and seminal plasma also triggered differential expression of the transcripts tested. Finally, our data imply that spermatozoa, seminal plasma components, and the act of mating induce differential mechanisms in the peri-ovulatory female reproductive tract, which are essential for tissue repair. Uterine homeostasis is maintained after mating by eliminating pathogens, foreign cells, and proteins by a transient inflammation of the uterus. Such inflammation does not occur in the oviductal sperm reservoir (utero-tubal junction, UTJ), colonized by a population of potentially fertile spermatozoa before the inflammatory changes are triggered. Semen entry (spermatozoa and/or seminal plasma) modifies the expression of regulatory genes, including cell proliferation and differentiation-related transcripts. Considering pigs display a fractionated ejaculation, this study aims to determine whether different ejaculate fractions differentially modulate cell proliferation and differentiation-related transcripts in the sow reproductive tract during the peri-ovulatory stage. Using species-specific microarray analyses, the differential expression of 144 cell proliferation and differentiation-related transcripts was studied in specific segments: cervix (Cvx), distal and proximal uterus (DistUt, ProxUt), UTJ, isthmus (Isth), ampulla (Amp), and infundibulum (Inf) of the peri-ovulatory sow reproductive tract in response to semen and/or seminal plasma cervical deposition. Most mRNA expression changes were induced by mating. In addition, while mating upregulates the fibroblast growth factor 1 (FGF1, p -value DistUt = 0. 0007; ProxUt = 0. 0253) transcript in the endometrium, both its receptor, the fibroblast growth factor receptor 1 (FGFR1, p -value DistUt = 2. 14 e −06 ; ProxUt = 0. 0027; UTJ = 0. 0458) transcript, and a potentiator of its biological effect, the fibroblast growth factor binding protein 1 (FGFBP1), were downregulated in the endometrium (p -value DistUt = 0. 0068; ProxUt = 0. 0011) and the UTJ (p -value UTJ = 0. 0191). The FGFBP1 was downregulated in the whole oviduct after seminal depositions (p -value Isth = 0. 0007; Amp = 0. 0007; Inf = 6. 87 e −05) and, interestingly, FGFR1 was downregulated in the endometrium in the absence of semen (p -value DistUt = 0. 0097; ProxUt = 0. 0456). In conclusion, the findings suggest that spermatozoa, seminal components, and the act of mating trigger, besides inflammation, differential mechanisms in the peri-ovulatory female reproductive tract, relevant for tissue repair.
Ayudas:	Ministerio de Ciencia e Innovación PID2019-108320RJ-I00 Ministerio de Ciencia e Innovación IJCI-2015-24380 Agència de Gestió d'Ajuts Universitaris i de Recerca 2018 FI_B 00236 Ministerio de Educación, Cultura y Deporte FPU15/06029
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Cell proliferation ; Cell differentiation ; Inflammation ; Interleukins ; Chemokines ; Fibroblast growth factors ; Insulin-like growth factors ; Pig
Publicado en:	Biology, Vol. 11 (april 2022) , ISSN 2079-7737

DOI: 10.3390/biology11040616
PMID: 35453814

16 p, 2.5 MB

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