Serum/plasma potassium monitoring using potentiometric point-of-care microanalyzers with improved ion selective electrodes
Calvo-Lopez, Antonio (Universitat Autònoma de Barcelona. Departament de Química)
Arasa Puig, Eva (Universitat Autònoma de Barcelona. Departament de Química)
Alonso Chamarro, Julián (Universitat Autònoma de Barcelona. Departament de Química)
Puyol Bosch, M. del Mar (Universitat Autònoma de Barcelona. Departament de Química)

Data:	2023
Resum:	Different causes can trigger imbalances on homeostatic mechanisms between intracellular and extracellular compartments resulting in abnormal blood potassium concentrations (hypo or hyperkalemia). This can lead to serious consequences, even a life-threatening situation. Early diagnosis, treatment and follow-up are essential to minimize critical impacts in patients. Bedside determination of blood potassium is not accessible in all health care centers or in all emergency departments and far less common in this kind of centers in emerging countries. We have therefore proposed a portable, economic and long-lifetime potentiometric point-of-care (POC) analytical microsystem to deal with this question. It is a continuous flow microfluidic platform, made of cyclic olefin copolymer (COC), which combines microfluidics and a detection system based on the potentiometric technique containing a potassium selective electrode with a novel composition of polymeric membrane, which improves lifetime. Its size is smaller than a credit card and shows a linear range of Nernst calibration equation from 1 to 26 mM K+, a detection limit of 0. 16 mM K+, a satisfactory repeatability and reproducibility, and an analysis frequency of 20 samples h-1, requiring only 25 μL as sample volume. Moreover, lifetime is as long as 9 months by intensive use. All these features comply with medical requirements. Human serum samples were analyzed with the developed device and the obtained results were compared with those provided by two methods: ICP-OES and another using ion selective electrodes. No significant differences were observed, demonstrating the suitability of the developed POC microanalyzer for bedside health applications.
Ajuts:	Agencia Estatal de Investigación CTQ2017-85011-R Agencia Estatal de Investigación PID2020-117216RB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-220
Nota:	Altres ajuts: acords transformatius de la UAB
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Biological samples ; Ion selective electrodes ; Lab on a chip ; Miniaturization ; Point-of-care ; Potassium
Publicat a:	Talanta, Vol. 253 (Feb. 2023) , art. 124100, ISSN 1873-3573

DOI: 10.1016/j.talanta.2022.124100

7 p, 1.4 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats