Web of Science: 3 cites, Scopus: 3 cites, Google Scholar: cites,
Metal-specificity divergence between Metallothioneins of Nerita peloronta (Neritimorpha, Gastropoda) sets the starting point for a novel chemical MT classification proposal
García-Risco, Mario (Universitat Autònoma de Barcelona. Departament de Química)
Calatayud, Sara (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Pedrini-Martha, Veronika (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences)
Albalat, Ricard (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Dallinger, Reinhard (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences)
Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química)
Capdevila Vidal, Mercè (Universitat Autònoma de Barcelona. Departament de Química)

Data: 2021
Resum: Metallothioneins' (MTs) biological function has been a matter of debate since their discovery. The importance to categorize these cysteine-rich proteins with high coordinating capacity into a specific group led to numerous classification proposals. We proposed a classification based on their metal-binding abilities, gradually sorting them from those with high selectivity towards Zn/Cd to those that are Cu-specific. However, the study of the NpeMT1 and NpeMT2isoforms of Nerita peloronta, has put a new perspective on this classification. N. peloronta has been chosen as a representative mollusk to elucidate the metal-binding abilities of Neritimorpha MTs, an order without any MTs characterized recently. Both isoforms have been recombinantly synthesized in cultures supplemented with ZnII, CdII, or CuII, and the purified metal-MT complexes have been thoroughly characterized by spectroscopic and spectrometric methods, leading to results that confirmed that Neritimorpha share Cd-selective MTs with Caenogastropoda and Heterobranchia, solving a so far unresolved question. NpeMTs show high coordinating preferences towards divalent metal ions, although one of them (NpeMT1) shares features with the so-called genuine Zn-thioneins, while the other (NpeMT2) exhibits a higher preference for Cd. The dissimilarities between the two isoforms let a window open to a new proposal of chemical MT classification.
Ajuts: Ministerio de Economía y Competitividad BIO2015-67358-C2-2-P
Ministerio de Economía y Competitividad BIO2015-67358-C2-1-P
Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-864
Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1665
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: Article ; recerca ; Versió publicada
Matèria: Biochemical classification ; Cd-selective ; Metallothioneins ; Neritimorpha
Publicat a: International journal of molecular sciences, Vol. 22, Issue 23 (December 2021) , art. 13114, ISSN 1422-0067

DOI: 10.3390/ijms222313114
PMID: 34884919


14 p, 2.0 MB

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 Registre creat el 2023-02-15, darrera modificació el 2023-02-19



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