Methicillin-Susceptible Staphylococcus aureus Biofilm Formation on Vascular Grafts : an In Vitro Study
Tello-Díaz, Cristina (Institut d'Investigació Biomèdica Sant Pau)
Palau Gauthier, Marta (Hospital Universitari Vall d'Hebron)
Muñoz, Estela (Hospital Universitari Vall d'Hebron)
Gomis, Xavier (Hospital Universitari Vall d'Hebron)
Gavaldà, Joan (Hospital Universitari Vall d'Hebron)
Fernández-Hidalgo, Nuria (Hospital Universitari Vall d'Hebron)
Bellmunt Montoya, Sergi (Hospital Universitari Vall d'Hebron)
Universitat Autònoma de Barcelona

Data:	2023
Resum:	The aim of this study was to quantify in vitro biofilm formation by methicillin-susceptible (MSSA) on the surfaces of different types of commonly used vascular grafts. We performed an in vitro study with two clinical strains of MSSA (MSSA2 and MSSA6) and nine vascular grafts: Dacron (Hemagard), Dacron-heparin (Intergard heparin), Dacron-silver (Intergard Silver), Dacron-silver-triclosan (Intergard Synergy), Dacron-gelatin (Gelsoft Plus), Dacron plus polytetrafluoroethylene (Fusion), polytetrafluoroethylene (Propaten; Gore), Omniflow II, and bovine pericardium (XenoSure). Biofilm formation was induced in two phases: an initial 90-minute adherence phase and a 24-hour growth phase. Quantitative cultures were performed, and the results were expressed as log CFU per milliliter. The Dacron-silver-triclosan graft and Omniflow II were associated with the least biofilm formation by both MSSA2 and MSSA6. MSSA2 did not form a biofilm on the Dacron-silver-triclosan graft (0 CFU/mL), and the mean count on the Omniflow II graft was 3. 89 CFU/mL (standard deviation [SD] 2. 10). The mean count for the other grafts was 7. 01 CFU/mL (SD 0. 82). MSSA6 formed a biofilm on both grafts, with 2. 42 CFU/mL (SD 2. 44) on the Dacron-silver-triclosan graft and 3. 62 CFU/mL (SD 2. 21) on the Omniflow II. The mean biofilm growth on the remaining grafts was 7. 33 CFU/mL (SD 0. 28). The differences in biofilm formation on the Dacron-silver-triclosan and Omniflow II grafts compared to the other tested grafts were statistically significant. Our findings suggest that of the vascular grafts we studied, the Dacron-silver-triclosan and Omniflow II grafts might prevent biofilm formation by MSSA. Although further studies are needed, these grafts seem to be good candidates for clinical use in vascular surgeries at high risk of infections due to this microorganism. IMPORTANCE The Dacron silver-triclosan and Omniflow II vascular grafts showed the greatest resistance to in vitro methicillin-susceptible biofilm formation compared to other vascular grafts. These findings could allow us to choose the most resistant to infection prosthetic graft.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Biofilm ; Infection ; In vitro ; Prosthesis infections ; Staphylococcus aureus ; Vascular graft
Publicat a:	Microbiology Spectrum, Vol. 11 (february 2023) , ISSN 2165-0497

DOI: 10.1128/spectrum.03931-22
PMID: 36749062

7 p, 744.9 KB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut de Recerca Sant Pau
Articles > Articles de recerca
Articles > Articles publicats