Characterization of the denaturation of bovine serum albumin (BSA) protein by means of a differential-mode microwave microfluidic sensor based on slot resonators
Muñoz Enano, Jonathan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Electrònica)
Peytral-Rieu, Olivia (Centre national de la recherche scientifique. Laboratoire d'analyse et d'architecture des systèmes)
Vélez Rasero, Paris (Universitat Autònoma de Barcelona. Departament d'Enginyeria Electrònica)
Dubuc, David (Centre national de la recherche scientifique. Laboratoire d'analyse et d'architecture des systèmes)
Grenier, Katia (Centre national de la recherche scientifique. Laboratoire d'analyse et d'architecture des systèmes)
Martín, Ferran (Martín Antolín) (Universitat Autònoma de Barcelona. Departament d'Enginyeria Electrònica)

Data:	2022
Resum:	This paper presents a differential-mode microwave microfluidic sensor useful for the characterization/detection of the denaturation of Bovine Serum Albumin (BSA), a protein with numerous biochemical applications, caused by a chaotropic agent, specifically, urea. The sensor consists of a pair of uncoupled microstrip lines, each one loaded with a transversally oriented slot resonator, etched in the ground plane. The sensor is a device able to detect permittivity changes in the so-called material under test (MUT), or liquid under test (LUT), as compared to a reference (REF) material/liquid. However, since the changes in the permittivity of the BSA caused by the denaturation process are partially obscured by the presence of the urea in the LUT, a protocol to separate the effects of the urea in the sample is proposed. According to such protocol, two different BSA samples, with different concentrations, are needed. It is shown that, with such a method, the denaturation process can be detected and characterized through the so-called hydration contrast, related to the measured difference of the output variables (the cross-mode transmission coefficient) in both BSA samples. The reported protocol and denaturation detection method based on differential-mode microwave sensing, which has never been reported so far, provides hydration contrasts of 0. 06 for 6M urea concentrations.
Ajuts:	Agencia Estatal de Investigación PDC2021-121085-I00 Agencia Estatal de Investigación PID2019-103904RB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1159
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Bovine serum albumin (BSA) ; Defect-ground-structure (DGS) ; Differential sensor ; Microfluidics ; Microwave sensor ; Protein denaturation ; Slot resonator
Publicat a:	IEEE sensors journal, Vol. 22, issue 14 (Juliol 2022) , p. 14075-14083, ISSN 1558-1748

DOI: 10.1109/JSEN.2022.3181542

Disponible a partir de: 2024-07-30 Postprint

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