Platelet-, monocyte-derived & tissue factorcarrying circulating microparticles are related to acute myocardial infarction severity
Chiva-Blanch, Gemma (Institut d'Investigació Biomèdica Sant Pau)
Laake, Kristian (University of Oslo)
Myhre, Peder (University of Oslo)
Bratseth, Vibeke (Oslo University Hospital Ullevål)
Arnesen, Harald (University of Oslo)
Solheim, Svein (Oslo University Hospital Ullevål)
Badimon, Lina (Institut d'Investigació Biomèdica Sant Pau)
Seljeflot, Ingejorg. (University of Oslo)
Universitat Autònoma de Barcelona

Data:	2017
Resum:	Circulating microparticles (cMPs) are phospholipid-rich vesicles released from cells when activated or injured, and contribute to the formation of intracoronary thrombi. Tissue factor (TF, CD142) is the main trigger of fibrin formation and TF-carrying cMPs are considered one of the most procoagulant cMPs. Similar types of atherosclerotic lesions may lead to different types of AMI, although the mechanisms behind are unresolved. Therefore, we aimed to investigate the phenotype of cMPs found in plasma of ACS patients and its relation to AMI severity and thrombotic burden. In a cross-sectional study, two hundred patients aged 75±4 years were included in the study 2±8 weeks after suffering an AMI. Annexin V positive (AV)-cMPs derived from blood and vascular cells were measured by flow cytometry. Plasma procoagulant activity (TF-PCA) was measured through a chromogenic assay. STEMI patients (n = 75) showed higher levels of platelet-derived cMPs [CD61/AV, CD31/ AV, CD42b/AV and CD31/CD42b/AV, P = 0. 048, 0. 038, 0. 009 and 0. 006, respectively], compared to NSTEMI patients (n = 125). Patients who suffered a heart failure during AMI (n = 17) had increased levels of platelet (CD61)-And monocyte (CD14)-derived cMPs carrying TF (CD142) (P<0. 0001 and 0. 004, respectively). Additionally, NYHA class III (n = 23) patients showed higher levels of CD142/AV, CD14/AV and CD14/CD142/AV cMPs than those in class I/II (P = 0. 001, 0. 015 and 0. 014, respectively). The levels of these cMPs positively correlated with TF-PCA (r≥0. 166, P≤0. 027, all). Platelets and monocytes remain activated in AMI patients treated as per guidelines and release cMPs that discriminate AMI severity. Therefore, TF-MPs, and platelet-And monocyte- MPs may reflect thrombotic burden in AMI patients.
Ajuts:	Ministerio de Economía y Competitividad CD13/00023
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	PloS one, Vol. 12 Núm. 2 (february 2017) , p. e0172558, ISSN 1932-6203

DOI: 10.1371/journal.pone.0172558
PMID: 28207887

12 p, 913.3 KB

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