Diagnostic value of genotypic analysis in primary cutaneous lymphomas using standardized biomed-2 polymerase chain reaction protocols : Experience in daily clinical practice
López Aventín, Daniel (Parc de Salut MAR de Barcelona)
Gallardo, Fernando (Parc de Salut MAR de Barcelona)
Colomo, Luis (Parc de Salut MAR de Barcelona)
Moragón, Ester (Parc de Salut MAR de Barcelona)
Vela, María Carmen (Parc de Salut MAR de Barcelona)
Duran-Jordà, Xavier (Institut Hospital del Mar d'Investigacions Mèdiques)
Bellosillo Paricio, Beatriz (Parc de Salut MAR de Barcelona)
Pujol Vallverdú, Ramón M (Parc de Salut MAR de Barcelona)
Universitat Autònoma de Barcelona

Data:	2021
Resum:	BIOMED-2 Concerted Action BMH4-CT98-3936 (BIO-MED-2) PCR protocols are an important diagnostic tool in the evaluation of cutaneous lymphomas. The aim of this study was to assess the diagnostic value of the genotyping results obtained by these techniques in daily clinical practice. A total of 360 paraffin-em-bedded skin samples were retrospectively reviewed from 114 cutaneous T-cell lymphomas and 35 cutaneous B-cell lymphomas. A total of 249 biopsies from 180 patients with benign lymphoid infiltrates served as controls. T-cell receptor and immunoglobulin gene rearrangements were assessed using the BIOMED-2 method. A combined T-cell receptor gamma and beta assay approach reliably distinguished cutaneous T-cell lymphomas from benign skin T-cell infiltrates (sen-sitivity 89. 4%; specificity 81. 5%). Analysis of com-plete immunoglobulin heavy chain rearrangements also differentiated cutaneous B-cell lymphomas from benign B-cell infiltrates (sensitivity 85. 7%; specificity 82. 4%). In conclusion, the full BIOMED-2 protocol is a useful aid combined with clinical, histological and immunophenotypical findings for assessment of lym-phoid clonality in skin lymphoid proliferations.
Ajuts:	Instituto de Salud Carlos III PT17/0015/0011
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	BIOMED-2 ; Clonality ; Cutaneous B-cell lymphoma ; Cutaneous T-cell lymphoma ; Multiplex polymerase chain reaction ; T-cell receptor genes ; Immunoglobulin genes
Publicat a:	Acta dermato-venereologica, Vol. 101 Núm. 5 (2021) , p. adv00460, ISSN 1651-2057

DOI: 10.2340/00015555-3828
PMID: 33954802

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