The Effect of cytoskeleton inhibitors on coccolith morphology in Coccolithus braarudii and Scyphosphaera apsteinii
Langer, Gerald (Marine Biological Association of the United Kingdom)
Probert, Ian (Station Biologique de Roscoff)
Cox, Madison B. (University of North Carolina Wilmington)
Taylor, Alison (University of North Carolina Wilmington)
Harper, Glenn M. (University of Plymouth)
Brownlee, Colin (University of Southampton)
Wheeler, Glen (Marine Biological Association of the United Kingdom)

Data:	2023
Resum:	The calcite platelets of coccolithophores (Haptophyta), the coccoliths, are among the most elaborate biomineral structures. How these unicellular algae accomplish the complex morphogenesis of coccoliths is still largely unknown. It has long been proposed that the cytoskeleton plays a central role in shaping the growing coccoliths. Previous studies have indicated that disruption of the microtubule network led to defects in coccolith morphogenesis in Emiliania huxleyi and Coccolithus braarudii. Disruption of the actin network also led to defects in coccolith morphology in E. huxleyi, but its impact on coccolith morphology in C. braarudii was unclear, as coccolith secretion was largely inhibited under the conditions used. A more detailed examination of the role of actin and microtubule networks is therefore required to address the wider role of the cytoskeleton in coccolith morphogenesis. In this study, we have examined coccolith morphology in C. braarudii and Scyphosphaera apsteinii following treatment with the microtubule inhibitors vinblastine and colchicine (S. apsteinii only) and the actin inhibitor cytochalasin B. We found that all cytoskeleton inhibitors induced coccolith malformations, strongly suggesting that both microtubules and actin filaments are instrumental in morphogenesis. By demonstrating the requirement for the microtubule and actin networks in coccolith morphogenesis in diverse species, our results suggest that both of these cytoskeletal elements are likely to play conserved roles in defining coccolith morphology.
Ajuts:	European Commission 670390
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Actin ; Calcification ; Coccolith ; Coccolithophore ; Cytoskeleton ; Microtubule
Publicat a:	Journal of Phycology, Vol. 59, Issue 1 (February 2023) , p. 87-96, ISSN 1529-8817

DOI: 10.1111/jpy.13303
PMID: 36380706

10 p, 1.4 MB

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