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| Pàgina inicial > Articles > Articles publicats > Interplay between coding and non-coding regulation drives the Arabidopsis seed-to-seedling transition |
| Data: | 2024 |
| Resum: | Translation of seed stored mRNAs is essential to trigger germination. However, when RNAPII re-engages RNA synthesis during the seed-to-seedling transition has remained in question. Combining csRNA-seq, ATAC-seq and smFISH in Arabidopsis thaliana we demonstrate that active transcription initiation is detectable during the entire germination process. Features of non-coding regulation such as dynamic changes in chromatin accessible regions, antisense transcription, as well as bidirectional non-coding promoters are widespread throughout the Arabidopsis genome. We show that sensitivity to exogenous ABSCISIC ACID (ABA) during germination depends on proximal promoter accessibility at ABA-responsive genes. Moreover, we provide genetic validation of the existence of divergent transcription in plants. Our results reveal that active enhancer elements are transcribed producing non-coding enhancer RNAs (eRNAs) as widely documented in metazoans. In sum, this study defining the extent and role of coding and non-coding transcription during key stages of germination expands our understanding of transcriptional mechanisms underlying plant developmental transitions. |
| Ajuts: | "la Caixa" Foundation 100010434 Ministerio de Economía y Competitividad SEV-2015-0533 Agencia Estatal de Investigación EUR2021-122003 Agencia Estatal de Investigación PID2019-110510GA-I00 Agencia Estatal de Investigación PRE2019-090156 |
| Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
| Llengua: | Anglès |
| Document: | Article ; recerca ; Versió publicada |
| Matèria: | Plant development ; Reprogramming ; Long non-coding RNAs ; Epigenetics |
| Publicat a: | Nature communications, Vol. 15 (February 2024) , art. 1724, ISSN 2041-1723 |
21 p, 3.0 MB |