A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real-time quantitative PCR
White, Helen E. (University of Southampton (Southampton, Regne Unit))
Deprez, Liesbet (University of Southampton (Regne Unit))
Corbisier, Philippe (Faculty of Medicine. University of Southampton (Regne Unit))
Hall, Victoria J. (Salisbury District Hospital)
Lin, Feng (University of Southampton (Regne Unit))
Mazoua, Stéphane (University of Southampton (Regne Unit))
Trapmann, Stefanie (University of Southampton (Regne Unit))
Aggerholm, Anni (Aarhus University Hospital (Aarhus, Dinamarca))
Andrikovics, Hajnalka (Hungarian National Blood Transfusion Service (Budapest, Hongria))
El Akiki, Susanna (Birmingham Women's NHS Foundation Trust (Birmingham, Regne Unit))
Barbany-Bustinza, Gisela (Clinical Genetics Karolinska Institutet (Suècia))
Boeckx, Nancy (KU Leuven (Lovaina, Bèlgica))
Bench, Anthony J. (Cambridge University Hospitals NHS Foundation Trust (Anglaterra))
Catherwood, Mark Alexander (Belfast City Hospital (Belfast, Irlanda))
Cayuela, Jean Michel (University Paris Diderot (París, França))
Chudleigh, Sandra (Yorkhill NHS Trust (Glasgow, Escòcia))
Clench, Tim (Bristol Royal Infirmary)
Colomer, Dolors (Hospital Clínic i Provincial de Barcelona)
Daraio, Filomena (University of Turin (Turin, Itàlia))
Dulucq, Stéphanie (Universite Bordeaux (Bordeus, França))
Farrugia, Joanna (Derriford Hospital (Plymouth, Regne Unit))
Fletcher, Linda (SA Pathology (Adelaida, Austràlia))
Foroni, Letizia (Imperial College London (Londres, Regne Unit))
Ganderton, Rosalind H. (University Hospital Southampton NHS Foundation Trust (Southampton, Regne Unit))
Gerrard, Gareth (Imperial College London (Londres, Regne Unit))
Gineikiene, Eglė (Vilnius University Hospital Santariskiu Clinics (Vilnius, Lituània))
Hayette, Sandrine (Hospices Civils de Lyon (Lió, França))
El Housni, Hakim (Erasme Hospital (Brussel·les, Bèlgica))
Izzo, Barbara (Università degli studi di Napoli Federico II)
Jansson, Mattias (Uppsala University (Uppsala, Suècia))
Johnels, Petra (University and Regional Laboratories (Lund, Suècia))
Jurcek, Tomas (Masaryk University and University Hospital Brno (Brno, República Txeca))
Kairisto, Veli (Turku University Hospital (Finlàndia))
Kizilors, Aytug (Kings College Hospital (Londres, Regne Unit))
Kim, Dong-Wook (Catholic University of Korea (Corea del Sud))
Lange, Thoralf (Universität Leipzig)
Lion, Thomas (Children's Cancer Research Institute (Viena, Àustria))
Machová Polakova, Katerina Machová (Institute of Hematology and Blood Transfusion (Praga, Txèquia))
Martinelli, Giovanni (University of Bologna (Bolonya, Itàlia))
Mccarron, S. (St James's Hospital (Irlanda))
Merle, Pauline A. (VU Medical Centre (Amsterdam, Països Baixos))
Milner, Benedict J. (NHS-Grampian (Aberdeen, Regne Unit))
Mitterbauer-Hohendanner, Gerlinde Juliane (Medical University of Vienna)
Nagar, Meital (Sheba Medical Center (Tel Aviv, Israel))
Nickless, Guillermina (Guy's and St Thomas' NHS Foundation Trust (Regne Unit))
Nomdedéu, Josep (Institut d'Investigació Biomèdica Sant Pau)
Nymoen, Dag André (Oslo University Hospital (Oslo, Noruega))
Oppliger Leibundgut, Elisabeth (University Hospital Bern (Berna, Suïssa))
Ozbek, Ugur (Istanbul University (Serdivan, Turquia))
Pajič, Tadej (University Medical Centre (Ljubljana, Eslovènia))
Pfeifer, Heike (Goethe University (Frankfurt, Alemanya))
Preudhomme, Claude (CHU Lille)
Raudsepp, K. (United Laboratories of Tartu University Hospitals (Tartu, Estònia))
Romeo, Giuliana (Royal Perth Hospital (Perth, Austràlia))
Sacha, Tomasz (Jagiellonian University (Krakow, Polònia))
Talmaci, R. (University of Medicine and Pharmacy 'Carol Davila' (Bucarest, Romania))
Touloumenidou, Tasoula (Papanicolaou Hospital (Thessaloniki, Grècia))
Van Der Velden, Vincent H. J. (Erasmus Medical Center)
Waits, Paula S. (Southmead Hospital (Bristol, Regne Unit))
Wang, Lihui (Royal Liverpool University Hospital (Liverpool, Regne Unit))
Wilkinson, Elizabeth (St James's University Hospital (Leeds, Regne Unit))
Wilson, Gillian A. (Sheffield Children's NHS Foundation Trust Londres, Regne Unit))
Wren, Dörte (The Royal Marsden NHS Foundation Trust)
Zadro, Renata (Zagreb University School of Medicine (Zagreb, Croàcia))
Ziermann, Janine (Jena University Hospital (Alemanya))
Zoi, Katerina (Academy of Athens)
Müller, Martin Christian (Medizinische Fakultät Mannheim der Universität Heidelberg (Mannheim, Alemanya))
Hochhaus, Andreas (Jena University Hospital (Alemanya))
Schimmel, Heinz G. (University of Southampton (Southampton, Regne Unit))
Cross, Nicholas C P. (University of Southampton (Southampton, Regne Unit))
Emons, Hendrik (University of Southampton (Southampton, Regne Unit))
Universitat Autònoma de Barcelona. Departament de Medicina

Data:	2015
Resum:	Serial quantification of BCR-ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukaemia, but there is a substantial variation in results reported by different laboratories. To improve comparability, an internationally accepted plasmid certified reference material (CRM) was developed according to ISO Guide 34:2009. Fragments of BCR-ABL1 (e14a2 mRNA fusion), BCR and GUSB transcripts were amplified and cloned into pUC18 to yield plasmid pIRMM0099. Six different linearised plasmid solutions were produced with the following copy number concentrations, assigned by digital PCR, and expanded uncertainties: 1. 08±0. 13 × 10 6, 1. 08±0. 11 × 10 5, 1. 03±0. 10 × 10 4, 1. 02±0. 09 × 10 3, 1. 04±0. 10 × 10 2 and 10. 0±1. 5 copies/μl. The certification of the material for the number of specific DNA fragments per plasmid, copy number concentration of the plasmid solutions and the assessment of inter-unit heterogeneity and stability were performed according to ISO Guide 35:2006. Two suitability studies performed by 63 BCR-ABL1 testing laboratories demonstrated that this set of 6 plasmid CRMs can help to standardise a number of measured transcripts of e14a2 BCR-ABL1 and three control genes (ABL1, BCR and GUSB). The set of six plasmid CRMs is distributed worldwide by the Institute for Reference Materials and Measurements (Belgium) and its authorised distributors (https://ec. europa. eu/jrc/en/reference-materials/catalogue/; CRM code ERM-AD623a-f).
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Calibration ; Cloning, Molecular ; DNA ; Escherichia coli Proteins ; Fusion Proteins, bcr-abl ; Gene Dosage ; Humans ; Membrane Transport Proteins ; Plasmids ; Proto-Oncogene Proteins c-bcr ; Real-Time Polymerase Chain Reaction ; Reference Standards ; RNA, Messenger
Publicat a:	Leukemia, Vol. 29 Núm. 2 (july 2015) , p. 369-376, ISSN 1476-5551

DOI: 10.1038/leu.2014.217
PMID: 25036192

8 p, 805.6 KB

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