The contribution of different sperm parameters to better explain ram semen cryoresistance
Yánez-Ortiz, Iván (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Garcia Vera, Wilber Calixto (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Tabarez, Abigail (Universidad Veracruzana)
Palomo Peiró, María Jesús (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Data:	2024
Resum:	In order to determine an effective procedure for explaining ram sperm cryoresistance and develop a new model for breeders classification, a retrospective study was conducted using sperm analysis data obtained over two consecutive years from a total of 82 sessions of ram semen cryopreservation. In each session, fresh ejaculates from eight males were collected via artificial vagina, pooled and frozen in liquid nitrogen vapors. After thawing, a total of 19,084 sperm tracks and 11,319 morphometric measurements were analysed. Clustering analyses were applied to establish motile and morphometric sperm subpopulations. Additionally, plasma and acrosome membrane integrity, as well mitochondrial activity using flow cytometry immediately after sperm thawing and following hypoosmotic shock test (HOST) was assessed. To develop a Ram Sperm Cryoresistance Index, Principal Component Analyses (PCA) using 22 variables were conducted. In the first PCA, the parameters that best explain cryoresistance include total motility (TM), motile subpopulation 2 (motSP2, which groups slow, very linear spermatozoa with low lateral head displacement), morphometric subpopulation 1 (morphSP1, grouping spermatozoa with the smallest head size and lowest shape values), sperm plasma membrane integrity immediately after thawing and following hypoosmotic shock test. These parameters collectively account for 77. 34 % of the accumulated variance. To emphasize their importance, a second PCA was performed, revealing significant higher weighting coefficients for the quantity (TM) and quality (motSP2) of sperm movement after thawing, compared to the head size and shape of the thawed sperm (morphSP1). Furthermore, HOST Viability played a more decisive role than what was observed under isotonic conditions.
Nota:	Altres ajuts: acords transformatius de la UAB
Nota:	Altres ajuts: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria RZ2009-00008-00-00. Secretary of Higher Education, Science, Technology and Innovation (SENESCYT), Ecuador CZ02-000507-2019
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Cryoresistance ; Ram sperm ; Principal components analysis
Publicat a:	The veterinary journal, Vol. 306 (august 2024) , p. 106194, ISSN 1532-2971

DOI: 10.1016/j.tvjl.2024.106194
PMID: 38986926

5 p, 374.5 KB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats