High corn oil and high extra virgin olive oil diets have different effects on the expression of differentiation-related genes in experimental mammary tumors
Moral Cabrera, Raquel (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Solanas i Garcia, Montserrat (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
García, Gemma (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Vela Sarrión, Elena (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Escrich Navarro, Raquel (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Escrich, Eduard (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Data:	2008
Resum:	Dietary lipids can modify the clinical behavior and morphological features of experimental breast tumors. We previously demonstrated that a high corn oil diet has a tumor-enhancing effect in 7,12-dimethylbenz(α)anthracene (DMBA)-induced rat mammary adenocarcinomas, whereas a high olive oil diet acts as a negative modulator of carcinogenesis. In this study, we investigated whether these high fat diets modulate the expression of genes related to differentiation. Rats were induced with DMBA and fed a low fat diet, a high corn oil diet, a high olive oil diet, or both high fat diets. The expression levels of the mammary differentiation biomarkers α-casein, β-casein and transferrin and of β-actin and its transporter zipcode binding protein 1 (ZBP1) were analyzed by Northern and/or Western blot in the mammary adenocarcinomas. The high fat diets did not induce changes in the expression of caseins, while transferrin expression was increased as a result of the high olive oil diet. β-actin mRNA levels were higher in the high fat diet groups, though no changes in the protein levels were observed. The expression of ZBP1, a protein reported as having a role in carcinogenesis, was significantly increased by the high corn oil diet. These results suggest that in this model caseins are not good biomarkers of the changes in tumor morphological differentiation conferred by the high fat diets. The modulation of transferrin and ZBP1 expression by the high olive oil and the high corn oil diets could be one of the mechanisms by which such diets have a different influence on mammary carcinogenesis.
Ajuts:	Ministerio de Ciencia, Innovación y Universidades BFI2003-07341 Ministerio de Ciencia, Innovación y Universidades AGL2006-07691/ALI
Nota:	Altres ajuts: Fundación Patrimonio Comunal Olivarero 2008-2012, FPCO2008-165396
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Llengua:	Anglès
Col·lecció:	Grau, Laura
Document:	Article ; recerca ; Versió publicada
Matèria:	Dietary lipids ; Breast cancer ; Differentiation ; Casein ; Transferrin ; ß-actin ; Zipcode binding protein 1
Publicat a:	Oncology Reports, Vol. 20 Núm. 2 (august 2008) , p. 429-435, ISSN 1791-2431

DOI: 10.3892/or_00000025

7 p, 2.1 MB

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