Adipose tissue-specific ablation of PGC-1β impairs thermogenesis in brown fat
Funda, Jiří (Charles University in Prague)
Villena, Josep A. (Vall d'Hebron Institut de Recerca (VHIR))
Bardova, Kristina (Institute of Physiology of the Czech Academy of Sciences)
Adamcova, Katerina (Institute of Physiology of the Czech Academy of Sciences)
Irodenko, Illaria (Institute of Physiology of the Czech Academy of Sciences)
Flachs, Pavel (Institute of Physiology of the Czech Academy of Sciences)
Jedlickova, Ivana (Charles University. Faculty of Medicine in Hradec Králové)
Haasova, Eliska (Charles University in Prague)
Rossmeisl, Martin (Institute of Physiology of the Czech Academy of Sciences)
Kopecky, Jan (Institute of Physiology of the Czech Academy of Sciences)
Janovska, Petra (Institute of Physiology of the Czech Academy of Sciences)

Data:	2022
Resum:	Impaired thermogenesis observed in mice with whole-body ablation of peroxisome proliferator-activated receptor-γ coactivator-1β (PGC-1β; officially known as PPARGC1B) may result from impaired brown fat (brown adipose tissue; BAT) function, but other mechanism(s) could be involved. Here, using adipose-specific PGC-1β knockout mice (PGC-1β-AT-KO mice) we aimed to learn whether specific PGC-1β ablation in adipocytes is sufficient to drive cold sensitivity. Indeed, we found that warm-adapted (30°C) mutant mice were relatively sensitive to acute cold exposure (6°C). When these mice were subjected to cold exposure for 7 days (7-day-CE), adrenergic stimulation of their metabolism was impaired, despite similar levels of thermogenic uncoupling protein 1 in BAT in PGC-1β-AT-KO and wild-type mice. Gene expression in BAT of mutant mice suggested a compensatory increase in lipid metabolism to counteract the thermogenic defect. Interestingly, a reduced number of contacts between mitochondria and lipid droplets associated with low levels of L-form of optic atrophy 1 was found in BAT of PGC-1β-AT-KO mice. These genotypic differences were observed in warm-adapted mutant mice, but they were partially masked by 7-day-CE. Collectively, our results suggest a role for PGC-1β in controlling BAT lipid metabolism and thermogenesis. This article has an associated with the first author of the paper. Summary: Adipose tissue-specific ablation of PGC-1β impairs thermogenesis in murine brown fat owing to a reduced number of contacts between mitochondria and lipid droplets, possibly involving the L-form of optic atrophy 1 protein.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Lipid metabolism ; OPA1 ; Mice ; Adrenergic control
Publicat a:	Disease Models & Mechanisms, Vol. 15 (april 2022) , ISSN 1754-8411

DOI: 10.1242/dmm.049223
PMID: 35466996

11 p, 3.7 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats