Antioxidized LDL antibodies are associated with different metabolic pathways in patients with atherosclerotic plaque and type 2 diabetes
Bernal-Lopez, María Rosa (Instituto de Salud Carlos III)
Garrido-Sanchez, Lourdes (Universitat Rovira i Virgili)
Gomez-Carrillo, Víctor (Hospital Regional Universitario Carlos Haya (Málaga))
Gallego-Perales, José Luis (Hospital Regional Universitario Carlos Haya (Málaga))
Llorente-Cortés, Vicenta (Institut d'Investigació Biomèdica Sant Pau)
Calleja, Fernando (Hospital Regional Universitario Carlos Haya (Málaga))
Gomez-Huelgas, Ricardo (Hospital Regional Universitario Carlos Haya (Málaga))
Badimon, Lina (Institut d'Investigació Biomèdica Sant Pau)
Tinahones, Francisco J. (Instituto de Salud Carlos III)
Universitat Autònoma de Barcelona

Data:	2013
Resum:	OBJECTIVE-Oxidized lipoproteins and antioxidized LDL antibodies (antioxLDLabs) have been detected in human plasma and atherosclerotic lesions. The principle aimof this study was to analyze the possible relationship between IgG and IgM antioxLDLabs and factors involved in different metabolic pathways (inflammation, lipid metabolism, apoptosis, and cell cycle arrest profile) in the occluded popliteal artery (OPA) compared with the femoral vein (FV). RESEARCH DESIGN AND METHODS-Fifteen patients with advanced atherosclerosis and type 2 diabetes undergoing lower limb amputation participated in this study. Each patient had OPA and FV biopsy specimens and peripheral arterial occlusive disease. By real-time PCR, gene expression was analyzed from the OPA and FV specimens, and antioxLDLab levels were measured by specific enzyme-linked immunosorbent assay. RESULTS-The OPA and FV showed a positive correlation between only IgM antioxLDLab levels and the expression of genes involved in different metabolic pathways, including inflammation (TFPI), apoptosis (BAX, caspase 3, AKT1), plaque disruption (MMP2 and MMP10), lipid metabolism (SCARB1, PPARg), and cell turnover (CDKN1A), and genes for transcription and growth factors (NFkB and VEGFA, respectively). CONCLUSIONS-The results show that gene expression in the metabolic pathways (apoptosis, lipid metabolism, and inflammation) in theOPA and FV are directly related to the levels of IgM antioxLDLabs. © 2013 by the American Diabetes Association.
Ajuts:	Ministerio de Sanidad y Consumo CB06/03/0018 Ministerio de Ciencia e Innovación JCI-2009-04086
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Diabetes Care, Vol. 36 Núm. 4 (april 2013) , p. 1006-1011, ISSN 1935-5548

DOI: 10.2337/dc12-1030
PMID: 23193212

6 p, 283.4 KB

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