The induction of cytokine release in monocytes by electronegative low-density lipoprotein (LDL) is related to its higher ceramide content than native LDL
Estruch, Montserrat (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Sanchez-Quesada, Jose Luis (Institut d'Investigació Biomèdica Sant Pau)
Beloki, Lorea (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Ordóñez, J. (Jordi) 1952- (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Benitez, Sonia (Institut d'Investigació Biomèdica Sant Pau)
Universitat Autònoma de Barcelona

Data:	2013
Resum:	Electronegative low-density lipoprotein (LDL(-)) is a minor modified LDL subfraction that is present in blood. LDL(-) promotes inflammation and is associated with the development of atherosclerosis. We previously reported that the increase of cytokine release promoted by this lipoprotein subfraction in monocytes is counteracted by high-density lipoprotein (HDL). HDL also inhibits a phospholipase C-like activity (PLC-like) intrinsic to LDL(-). The aim of this work was to assess whether the inhibition of the PLC-like activity by HDL could decrease the content of ceramide (CER) and diacylglycerol (DAG) generated in LDL(-). This knowledge would allow us to establish a relationship between these compounds and the inflammatory activity of LDL(-). LDL(-) incubated at 37 °C for 20 h increased its PLC-like activity and, subsequently, the amount of CER and DAG. We found that incubating LDL(-) with HDL decreased both products in LDL(-). Native LDL was modified by lipolysis with PLC or by incubation with CER-enriched or DAG-enriched liposomes. The increase of CER in native LDL significantly increased cytokine release, whereas the enrichment in DAG did not show these inflammatory properties. These data point to CER, a resultant product of the PLC-like activity, as a major determinant of the inflammatory activity induced by LDL(-) in monocytes. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
Ajuts:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-1205
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Ceramide ; Cytokines ; Diacylglycerol ; Electronegative LDL ; HDL ; Lipoprotein aggregation ; Monocytes ; Phospholipase C
Publicat a:	International journal of molecular sciences, Vol. 14 Núm. 2 (february 2013) , p. 2601-2616, ISSN 1422-0067

DOI: 10.3390/ijms14022601
PMID: 23358250

16 p, 1.2 MB

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