Chromosomal inversions promote genomic islands of concerted evolution of Hsp70 genes in the Drosophila subobscura species subgroup
Puig Giribets, Marta (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
García Guerreiro, María Pilar (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Santos, Mauro (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Ayala, Francisco J. 1934- (University of California. Department of Ecology and Evolutionary Biology)
Tarrío, Rosa (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Rodríguez-Trelles, Francisco (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data:	2019
Resum:	Heat-shock (HS) assays to understand the connection between standing inversion variation and evolutionary response to climate change in Drosophila subobscura found that "warm-climate" inversion O 3+4 exhibits non-HS levels of Hsp70 protein like those of "cold-climate" O ST after HS induction. This was unexpected, as overexpression of Hsp70 can incur multiple fitness costs. To understand the genetic basis of this finding, we have determined the genomic sequence organization of the Hsp70 family in four different inversions, including O ST , O 3+4 , O 3+4+8 and O 3+4+16 , using as outgroups the remainder of the subobscura species subgroup, namely Drosophila madeirensis and Drosophila guanche. We found (i) in all the assayed lines, the Hsp70 family resides in cytological locus 94A and consists of only two genes, each with four HS elements (HSEs) and three GAGA sites on its promoter. Yet, in O ST, the family is comparatively more compact; (ii) the two Hsp70 copies evolve in concert through gene conversion, except in D. guanche; (iii) within D. subobscura, the rate of concerted evolution is strongly structured by inversion, being higher in O ST than in O 3+4 ; and (iv) in D. guanche, the two copies accumulated multiple differences, including a newly evolved "gap-type" HSE2. The absence of concerted evolution in this species may be related to a long-gone-unnoticed observation that it lacks Hsp70 HS response, perhaps because it has evolved within a narrow thermal range in an oceanic island. Our results point to a previously unrealized link between inversions and concerted evolution, with potentially major implications for understanding genome evolution.
Ajuts:	Ministerio de Economía y Competitividad CGL2013-42432P Agencia Estatal de Investigación CGL2017-89160P Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-636 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1379
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Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Chromosomal inversion polymorphism ; Climate change ; Concerted evolution ; Drosophila subobscura subgroup ; Evolution on islands ; Hsp70 ; SDG 13 - Climate Action
Publicat a:	Molecular ecology, Vol. 28, Issue 6 (March 2019) , p. 1316-1332, ISSN 1365-294X

DOI: 10.1111/mec.14511
PMID: 29412486

Postprint 49 p, 1.1 MB

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