Prepubertal goat oocytes from large follicles result in similar blastocyst production and embryo ploidy than those from adult goats
Romaguera i Matas, Roser (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Moll, Xavier (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Morató, Roser (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Roura, Montserrat (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Palomo Peiró, María Jesús (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Catalá, Maria-Gracia (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Jiménez y de Macedo, Ana Raquel (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Hammami, Sondes (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Izquierdo Tugas, Ma. Dolors (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Mogas Amorós, Teresa (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Paramio, María Teresa (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Data:	2011
Resum:	Developmental competence of oocytes from prepubertal females is lower than those from adult females. Oocyte development competence is positively related to follicular diameter. Most of the follicles of prepubertal goat ovaries are smaller than 3 mm. The aim of this study was to compare oocytes of two follicle sizes (< 3 mm and ≥ 3 mm) from prepubertal goats with oocytes from adult goats in relation to their in vitro production and quality of blastocysts. Oocytes from prepubertal goats were obtained from slaughterhouse ovaries and selected according to the follicle diameter whereas oocytes from adult goats were recovered in vivo by LOPU technique without prior selection of follicle size. COCs were IVM for 27 h, IVF at the conventional conditions with fresh semen and presumptive zygotes were cultured in SOF medium for 8 days. Blastocysts obtained were vitrified and after warming their blastocoele re-expansion and the ploidy by FISH technique were assessed. We found significant differences between blastocysts yield of oocytes recovered from follicles smaller than 3 mm of prepubertal goats compared to those from adult goats (5. 45% vs 20. 83%, respectively) however, these differences disappear if oocytes were recovered form large follicles (18. 07%). A total of 28 blastocysts were analysed and 96. 43% showed mixoploidy. Age did not affect the number of embryos with abnormal ploidy or blastocyst re-expansion after warming. Furthermore, the percentage of diploid blastomeres per embryo was similar in the 3 groups studied, adult, prepubertal from follicles ≥ 3 mm and < 3 mm (68. 6%, 80. 8% and 73. 6%, respectively). In conclusion, IVP of blastocysts coming from follicles larger than 3 mm of goats 45 days old were not different to the blastocysts produced from adult goats, both in terms of quantity and quality. © 2011 Elsevier Inc.
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Llengua:	Anglès
Document:	Article de revisió ; recerca ; Versió sotmesa a revisió
Matèria:	FISH ; Follicle size ; Goat ; IVEP ; Prepubertal
Publicat a:	Theriogenology, Vol. 76 Núm. 1 (2011) , p. 1-11, ISSN 1879-3231

DOI: 10.1016/j.theriogenology.2010.12.014

34 p, 453.4 KB

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