Hepatic gene transfer in animals using retroviruses containing the promoter from the gene for phosphoenolpyruvate carboxykinase
Hatzoglou, Maria (Case Western Reserve University School of Medicine (Cleveland, Estats Units d'Amèrica))
Lamers, Wouter
Bosch i Tubert, Fàtima (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Wynshaw-Boris, Anthony (Case Western Reserve University School of Medicine (Cleveland, Estats Units d'Amèrica))
Clapp, D. Wade (Case Western Reserve University School of Medicine (Cleveland, Estats Units d'Amèrica))
Hanson, Richard W. (Case Western Reserve University School of Medicine (Cleveland, Estats Units d'Amèrica))

Data:	1990
Resum:	Two methods are described for directing the expression of genes to the livers of animals using retroviral vectors containing the predominantly liver-specific promoter from the gene for phosphoenolpyruvate carboxykinase (PEPCK)-linked to the structural gene for either amino 3'-glycosyl phosphotransferase (neo) or bovine growth hormone (bGH). Replication-incompetent retrovirus was used to infect the livers of fetal rats by intraperitoneal injection of animals in utero or to infect adult rats by direct injection into the portal vein after partial hepatectomy. The proviruses were integrated into the hepatic DNA, and the chimeric genes were expressed from the PEPCK promoter for as long as 8 months after infection. The expression of the PEPCK-bGH gene was regulated by diet and hormones in a manner similar to the regulation of the endogenous PEPCK gene in the liver. The potential of this method for targeting genes to the liver is discussed.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Journal of biological chemistry, Vol. 265 Núm. 28 (1990) , p. 17285-17293, ISSN 1083-351X

9 p, 4.4 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats