Can immunocrit be used as a monitoring tool for swine vaccination and infection studies?
Sagrera, Mònica (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Sibila Vidal, Rosa Marina (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Martínez-Boixaderas, Núria (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Llorens, Anna Maria (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Espigares, David (Ceva Salud Animal)
Pastor Milán, Josep (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Garza-Moreno, Laura (Ceva Salud Animal)
Segalés Coma, Joaquim (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals

Data:	2024
Resum:	Background: The immunocrit is a cost-effective and straightforward technique traditionally used to assess passive immunity transfer to newborn piglets. However, it has not been previously used for monitoring the effect of vaccination and/or infections. Therefore, this study aimed to evaluate the usefulness of the immunocrit technique as an immunological monitoring tool in a vaccination and challenge scenario, using porcine circovirus 2 (PCV-2) as pathogen model. The immunocrit ratio was monitored in PCV-2 vaccinated (V) and non-vaccinated (NV) 3-week-old piglets (study day 0, SD0) that were subsequently challenged with this virus at SD21 and followed up to SD42. Additional techniques (PCV-2 IgG ELISA, optical refractometry, and proteinogram) were performed to further characterize the results of the immunocrit analysis. Results: Immunocrit, γ-globulin concentration and PCV-2 S/P values followed similar dynamics: descending after PCV-2 vaccination but ascending after an experimental PCV-2 inoculation. However, statistically significant differences between V and NV animals were only found with the PCV-2 ELISA. In this case, V animals had significantly higher (p < 0. 05) S/P values (S/P ratio = 0. 74) than NV (S/P ratio = 0. 39) pigs only after challenge at SD42. On the other hand, serum total protein obtained by refractometer (STPr) were maintained from SD0 to SD21 and increased in both groups from SD21 to SD42. Correlations between techniques were low to moderate, being the most robust ones found between immunocrit and optical refractometry (ρ = 0. 41) and immunocrit with γ-globulins (ρ = 0. 39). In a subset of sera, the proteinogram technique was applied to the whole serum and the supernatant of the immunocrit, with the objective to characterize indirectly the immunocrit fraction. The latter one included all protein types detectable through the proteinogram, with percentages varying between 64. 3% (γ-globulins) and 82% (β-globulins). Conclusion: The immunocrit technique represented a fraction of the total serum proteins, with low to moderate correlation with all the complementary techniques measured in this study. Its determination at different time points did not allow monitoring the effect of vaccination and/or infection using PCV-2 as a pathogen model.
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Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Immunocrit ; Infection ; Monitoring ; Porcine circovirus 2 ; Total serum protein ; Vaccination ; SDG 3 - Good Health and Well-being
Publicat a:	Porcine health management, Vol. 10 Núm. 1 (2024) , p. 30, ISSN 2055-5660

DOI: 10.1186/s40813-024-00380-y
PMID: 39180120

9 p, 1.4 MB

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