CD66b+/CD68+ circulating extracellular vesicles, lactate dehydrogenase and neutrophil-to-lymphocyte ratio can differentiate coronavirus disease 2019 severity during and after infection
Suades, Rosa (Institut de Recerca Sant Pau)
Greco, M.F. (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Prieto, P. (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Padró, Teresa (Institut de Recerca Sant Pau)
Devaux, Yvan (Luxembourg Institute of Health)
Domingo, Pere (Institut de Recerca Sant Pau)
Badimon, Lina (Institut de Recerca Sant Pau)
Universitat Autònoma de Barcelona

Data:	2024
Resum:	Coronavirus disease 2019 (COVID-19) has been a major public health burden. We hypothesised that circulating extracellular vesicles (cEVs), key players in health and disease, could trace the cell changes during COVID-19 infection and recovery. Therefore, we studied the temporal trend of cEV and inflammatory marker levels in plasma samples of COVID-19 patients that were collected within 24 h of patient admission (baseline, n = 80) and after hospital discharge at day-90 post-admission (n = 59). Inflammatory markers were measured by standard biochemical methods. cEVs were quantitatively and phenotypically characterized by high-sensitivity nano flow cytometry. In patients recovered from COVID-19 lower levels of inflammatory markers were detected. cEVs from vascular (endothelial cells) and blood (platelets, distinct immune subsets) cells were significantly reduced at day-90 compared to admission levels, a pattern also observed for cEVs from progenitor, perivascular and epithelial cells. The best discriminatory power for COVID-19 severity was found for inflammatory markers lactate dehydrogenase and neutrophil-to-lymphocyte ratio and for granulocyte/macrophage-released CD66b/CD68-cEVs. Albeit inflammatory markers were good indicators of systemic inflammatory response and discriminators of COVID-19 remission, they do not completely reveal cell stress and organ damage states. cEVs reaching baseline pre-infection levels at 90 days post-infection in recovered patients discriminate parental cells affected by disease.
Ajuts:	Agencia Estatal de Investigación PID2019-107160RB-I00 Ministerio de Economía y Competitividad CB16/11/00411 Instituto de Salud Carlos III PI19/01687
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Coronavirus disease 2019 ; Extracellular vesicles ; Inflammatory markers ; Microvesicles ; Severe acute respiratory syndrome coronavirus-2
Publicat a:	Journal of extracellular vesicles, Vol. 13 Núm. 7 (july 2024) , p. e12456, ISSN 2001-3078

DOI: 10.1002/jev2.12456
PMID: 39007437

15 p, 1.7 MB

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