Frozen dispersions of nanomaterials are a useful operational procedure in nanotoxicology
Vila Vecilla, Laura (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Rubio Lorente, Laura (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Annangi, Balasubramanyam (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
García Rodríguez, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Hernández Bonilla, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data:	2017
Descripció:	10 pàg.
Resum:	The variability observed in nanoparticle (NP) dispersions can affect the reliability of the results obtained in short-term tests, and mainly in long-term experiments. In addition, obtaining a good dispersion is time-consuming and acts as a bottleneck in the development of high-throughput screening methodologies. The freezing of different aliquots from a stock dispersion would overcome such limitations, but no studies have explored the impact of freezing thawing the samples on the physico-chemical and biological properties of the nanomaterial (NM). This work aims to compare fresh-prepared and frozen MWCNT, ZnO-, Ag-, TiO2- and CeO2-NP dispersions, used as models. NP characterization (size and morphology by TEM), hydrodynamic size and zeta potential were performed. Viability comparisons were determined in BEAS-2B cells. Cellular NP uptake and induced ROS production was assessed by TEM and flow cytometry, respectively. The obtained results show no important differences between frozen and fresh NP in their physico-chemical characteristics or their biological effects. This study is the first to demonstrate that there is no scientific evidence to dismiss the use of frozen NP, opening the door to the development of short- and long-term experiments with higher consistency, accuracy and reproducibility in a much shorter time and using a simplified procedure.
Ajuts:	European Commission 310584 Ministerio de Economía y Competitividad SAF2015-63519-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-202
Drets:	Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Ag-NP ; Beas-2b ; CeO2-NP ; Fresh and frozen nanomaterials ; Mwcnt ; TiO2-NP ; ZnO-NP ; Biological properties
Publicat a:	Nanotoxicology, Vol. 11, issue 1 (2017) , p. 31-40, ISSN 1743-5404

DOI: 10.1080/17435390.2016.1262918
PMID: 27855561

Postprint 34 p, 1.1 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats