Effects of different vaccination regimes on the immunodiagnosis of tuberculosis in goats and evaluation of defined antigens
Cuenca Lara, Patricia (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Blay-Benach, Miriam (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Cervera, Zoraida (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Melgarejo, Cristian (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Moraleda, Julia (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Sevilla, Iker A. (NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario. Animal Health Department)
Garrido, Joseba M. (NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario. Animal Health Department)
Singh, Mahavir (LIONEX Diagnostics and Therapeutics GmbH)
Jones, Gareth J. (Animal and Plant Health Agency. Department of Bacteriology)
Pérez de Val, Bernat (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)

Data:	2025
Resum:	Tuberculosis (TB) in goats is a chronic infectious disease caused by Mycobacterium tuberculosis complex (MTBC) organisms that pose a great health and economic challenge for the caprine industry in some European and developing countries. It is also a zoonotic disease posing a risk for public health. The control programs of the disease are based on a test-and-slaughter strategy, and vaccination is not feasible with available vaccines due to its interferences with the current TB immunodiagnosis. There is still a need for the development of an effective TB vaccine and, concurrently, diagnostic methods that allow differentiation between infected and vaccinated animals (DIVA approach). In this study, we investigated the interferences caused by the tuberculin (PPD)-based TB diagnostic tests in goats immunized by different mucosal and parenteral vaccination strategies: three single-dose strategies based on intranasal administration of BCG and two heat-inactivated M. bovis (HIMB) vaccines, and two prime-boost strategies based on parenteral BCG or HIMB priming and intranasal HIMB boosting. In addition, the defined antigens ESAT-6, CPF10, and EspC were evaluated as alternative diagnostic reagents to PPDs. At week 14 after prime vaccination of the animals, skin tests, IFN- γ release assay, and antibody detection assays were performed. The two prime-boosted and the single-dose intranasal BCG groups displayed greater cell-mediated immune responses to PPDs than the two single-dose intranasal HIMB vaccines. However, the use of reagents based on the defined antigens eliminated or reduced the vaccine-induced diagnostic interferences in all groups. Based on these results, the use of defined antigens in the current immunodiagnostic tests appears to be suitable in a future goat TB vaccination scenario.
Ajuts:	Ministerio de Ciencia e Innovación PID2022-142939OR-C22 European Commission INNOTUB II - EFA115/01
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Vaccines ; Mycobacterium bovis ; Tuberculosis ; Diagnosis ; Skin test ; IGRA ; DIVA
Publicat a:	Frontiers in veterinary science, Vol. 11 (january 2025) , ISSN 2297-1769

DOI: 10.3389/fvets.2024.1524461
PMID: 39931351

9 p, 2.4 MB

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