Testing of a novel automated point-of-care analyzer for blood ammonium monitoring in a clinical setting
Rebollo Calderón, Beatriz (Universitat Autònoma de Barcelona. Departament de Química)
Calvo-Lopez, Antonio (Institut de Recerca Sant Joan de Déu)
Ormazábal, Aida (Institut de Recerca Sant Joan de Déu)
Artuch, R (Institut de Recerca Sant Joan de Déu)
Rosell Ferrer, Javier (Universitat Politècnica de Catalunya)
Alonso Chamarro, Julián (Universitat Autònoma de Barcelona. Departament de Química)
Puyol Bosch, M. del Mar (Universitat Autònoma de Barcelona. Departament de Química)

Data:	2025
Resum:	Certain diseases are marked by elevated ammonium levels in the blood, a condition known as hyperammonemia. Prompt detection and medical intervention are crucial to prevent potentially fatal outcomes. Therefore, ammonium levels should be monitored regularly, typically in referral hospitals where specialized and costly equipment is available. Although compact commercial devices are available for this purpose, none of them meet all the technical and analytical requirements needed for direct blood analysis, and current reported strategies have not been validated with enough samples to confirm results reliably. We present a robust and reliable automated point-of-care (POC) analyzer for the potentiometric determination of ammonium in blood. Comprising three computer-controlled modules-fluid management, detection, and data acquisition and transmission-this system combines portability, ease of use, and affordability. It can directly measure untreated blood samples, significantly reducing analysis time. Fully automated, it operates unsupervised with minimal lab personnel intervention. Analytical quality parameters include 5% RSD repeatability (n = 8), a limit of detection of 24 μM, a working range of 30-1000 µM and a sample volume of 215 µL. Successfully implemented in a hospital for 2 months, it analyzed 238 blood samples in parallel with the hospital's reference method showing comparable results (paired t-test, Passing-Bablok regression and Bland-Altman Plot) and randomly distributed errors, with a 4% accuracy calculated as mean error. Results indicate the POC analyzer effectiveness and reliability in a clinical setting compared to currently reported or commercially available equipment, being suitable for bedside monitoring of conditions associated with hyperammonemia in healthcare centers, including emergency rooms and clinics in developing countries.
Ajuts:	Instituto de Salud Carlos III DTS18/00104 Instituto de Salud Carlos III DTS18/00075 Agencia Estatal de Investigación CTQ2017-85011-R Agencia Estatal de Investigación PID2020-117216RB Ministerio de Ciencia e Innovación PDC2021-121558-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00124
Nota:	Altres ajuts: acords transformatius de la UAB
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Potentiometry ; Hyperammonemia ; Inborn error of metabolism ; Point-of-care ; Ammonium selective electrodes
Publicat a:	Analytical and bioanalytical chemistry, (April 2025) , ISSN 1618-2650

DOI: 10.1007/s00216-025-05879-z
PMID: 40266320

9 p, 1.3 MB

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