Challenges and advances towards the rational design of microalgal synthetic promoters in Chlamydomonas reinhardtii
Milito, Alfonsina (Centre de Recerca en Agrigenòmica)
Aschern, Moritz (Centre de Recerca en Agrigenòmica)
Mcquillan, Josie L . (University of Sheffield. Department of Chemical and Biological Engineering)
Yang, Jae-Seong (Centre de Recerca en Agrigenòmica)

Data:	2023
Resum:	Microalgae hold enormous potential to provide a safe and sustainable source of high-value compounds, acting as carbon-fixing biofactories that could help to mitigate rapidly progressing climate change. Bioengineering microalgal strains will be key to optimizing and modifying their metabolic outputs, and to render them competitive with established industrial biotechnology hosts, such as bacteria or yeast. To achieve this, precise and tuneable control over transgene expression will be essential, which would require the development and rational design of synthetic promoters as a key strategy. Among green microalgae, Chlamydomonas reinhardtii represents the reference species for bioengineering and synthetic biology; however, the repertoire of functional synthetic promoters for this species, and for microalgae generally, is limited in comparison to other commercial chassis, emphasizing the need to expand the current microalgal gene expression toolbox. Here, we discuss state-of-the-art promoter analyses, and highlight areas of research required to advance synthetic promoter development in C. reinhardtii. In particular, we exemplify high-throughput studies performed in other model systems that could be applicable to microalgae, and propose novel approaches to interrogating algal promoters. We lastly outline the major limitations hindering microalgal promoter development, while providing novel suggestions and perspectives for how to overcome them.
Ajuts:	Agencia Estatal de Investigación PRE2020-092845 Agencia Estatal de Investigación CEX2019-000902-S Agencia Estatal de Investigación PID2020-117772GA-I00 Agencia Estatal de Investigación RYC2020-028880-I European Commission 945043
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Llengua:	Anglès
Document:	Article de revisió ; recerca ; Versió acceptada per publicar
Matèria:	Bioengineering ; Bioinformatics ; Chlamydomonas ; High-throughput approaches ; Microalgae ; Nuclear gene expression ; Promoter ; Synthetic biology ; Transcriptional regulation
Publicat a:	Journal of experimental botany, Vol. 74, Issue 13 (July 2023) , p. 3833-3850, ISSN 1460-2431

DOI: 10.1093/jxb/erad100

Postprint 49 p, 445.4 KB

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