Brachytherapy for targeting the immune system in cervical cancer patients
Linares, Isabel (Institut d'Investigació Biomèdica de Bellvitge)
Berenguer Frances, Miguel Ángel (Hospital Universitari i Politècnic La Fe (València))
Cañas, Rut (Institut d'Investigació Biomèdica de Bellvitge)
Najjari, Dina (Institut Català d'Oncologia)
Gutiérrez, Cristina (Institut Català d'Oncologia)
Marín, Susanna (Institut Català d'Oncologia)
Comas, Silvia (Institut Català d'Oncologia)
Guedea, Ferran (Institut d'Investigació Biomèdica de Bellvitge)
Pujol Canadell, Mònica (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)

Data:	2023
Resum:	New combinations based on standard therapeutic modalities and immunotherapy require understanding the immunomodulatory properties of traditional treatments. The objective was to evaluate the impact of brachytherapy (BT) on the immune system of cervical cancer and to identify the best modality, High-dose-rate brachytherapy (HDR-BT) vs. Pulsed-dose-rate (PDR-BT), to target it. Nineteen patients enrolled in a prospective study received chemoradiation (CRT) and subsequently HDR-BT or PDR-BT. Peripheral blood samples were obtained for immunophenotyping analysis by flow-cytometry before CRT, BT, and two and four weeks after BT. The Friedman one-way ANOVA, Conover post hoc test, and the Wilcoxon signed-rank test were used to compare changes in cell populations at different periods, perform multiple pairwise comparisons and assess differences between treatment groups (PDR and HDR). Natural killer cells (NKs) were the best target for BT. Patients receiving HDR-BT achieved significantly higher values ​​and longer time of the CD56dimCD16 + NK cells with greater cytotoxic capacity than the PDR-BT group, which presented their highest elevation of CD56-CD16 + NK cells. Furthermore, both BT modalities were associated with an increase in myeloid-derived suppressor cells (MDSCs), related to a worse clinical prognosis. However, there was a decrease in the percentage of CD4 + CD25 + Foxp3 + CD45RA + regulatory T cells (Tregs) in patients receiving HDR-BT, although there were no significant differences between BT. Immune biomarkers are important predictive determinants in cervical cancer. Higher cytotoxic NK cells and a trend toward lower values of Tregs might support the use of HDR-BT to the detriment of PDR-BT and help develop effective combinations with immunotherapy. The online version contains supplementary material available at 10. 1186/s12865-023-00559-y.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Immune system ; Cervical cancer ; Brachytherapy, Flow cytometry ; Natural killer cells ; Immunosuppressive cells
Publicat a:	BMC Immunology, Vol. 24 (August 2023) , art. 23, ISSN 1471-2172

DOI: 10.1186/s12865-023-00559-y
PMID: 37559025

11 p, 1.5 MB

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