Serum and urinary monocyte chemoattractant protein-1 as markers of inflammation and renal damage in dogs with naturally occurring leishmaniosis
Pantaleo, Valeria (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Furlanello, Tommaso (San Marco Veterinary Clinic and Laboratory)
Ventura, Laura (Università di Padova)
Solano Gallego, Laia (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Data:	2024
Resum:	Renal disease in canine leishmaniosis is of great importance owing to increased risk of mortality. In human visceral leishmaniosis, monocyte chemoattractant protein-1 (MCP-1) has been used as a marker of renal damage and inflammation. The purpose of this study was first to determine the serum MCP-1 and urinary MCP-1-to-creatinine ratio (uMCP-1/Cr) in healthy dogs and dogs with leishmaniosis at diagnosis, and second to determine whether these markers can differentiate disease severity at diagnosis. In total, 19 healthy seronegative dogs and 38 dogs with leishmaniosis were included in the study. Dogs with leishmaniosis were classified as LeishVet clinical staging and as International Renal Interest Society (IRIS) staging. Serum and urinary MCP-1 concentrations were measured with an enzyme-linked immunosorbent assay. A receiver operating characteristic (ROC) curve determined disease severity at diagnosis between two LeishVet groups (Stage II versus stage III and IV). Dogs in Leishvet stages IIb, III, and IV had a median serum MCP-1 and uMCP-1/Cr concentration higher than healthy dogs (P < 0. 0001). No statistical differences were found in serum MCP-1 and uMCP-1/Cr between dogs in LeishVet stage IIa and healthy dogs. The dogs in LeishVet stage IV had significantly higher serum MCP-1 and uMCP-1/Cr compared with the dogs in LeishVet stage IIa (P < 0. 0001). Serum MCP-1 and uMCP-1 were significantly higher in dogs in IRIS stage I and II + III + IV compared with healthy dogs. Dogs stage II + III + IV of IRIS had a significantly higher serum MCP-1 compared with dogs in IRIS stage I (P < 0. 0001). The area under the ROC curve for serum MCP-1 was 0. 78 [95% confidence interval (CI) 0. 64-0. 93] and for uMCP-1/Cr it was 0. 86 (95% CI, 0. 74-0. 99). The optimal cutoff value for serum MCP-1 and uMCP-1/Cr was 336. 85 pg/ml (sensitivity of 79% and specificity of 68%) and 6. 89 × 10 -7 (sensitivity of 84% and specificity of 79%), respectively. Serum MCP-1 and uMCP-1/Cr are increased in dogs with leishmaniosis compared with healthy dogs, suggesting the presence of inflammation and renal injury. Serum MCP-1 and uMCP-1/Cr were more elevated in the advanced stages of the disease compared with the moderate stages and, therefore, can be markers of the severity of the disease process. The online version contains supplementary material available at 10. 1186/s13071-024-06432-0.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Canine ; Leishmania infantum ; Renal disease ; LeishVet ; IRIS ; Clinical staging
Publicat a:	Parasites & vectors, Vol. 17 (august 2024) , ISSN 1756-3305

DOI: 10.1186/s13071-024-06432-0
PMID: 39210379

14 p, 1.0 MB

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